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Training and Documentation

Up-to-date inventory maintained for all hazardous materials?

Chemical Safety Data Sheets (SDS) maintained and readily available at all times employees are present?

Workplace hazard assessment and certification completed?

Lab workers received institutional safety training (typically provided by Environmental Health and Safety office) and supplemental laboratory-specific safety training for the hazards present in the laboratory?

Lab workers are familiar with physical and health hazards of chemicals in work area?

Lab workers able to describe how to detect the presence or release of hazardous materials?

Lab workers know how to protect themselves and others from effects of hazardous materials?

Chemical hygiene plan readily available and laboratory workers are familiar with it?

Spill and Emergency Planning

Lab workers familiar with the fire safety and building evacuation procedures including evacuation routes, nearest fire exits, fire alarm pull stations, and fire extinguishers?

Emergency procedures and phone numbers clearly posted?

First aid materials readily available and up-to-date?

Required "antidotes" or special first aid materials available (e.g., hydrofluoric acid requires calcium gluconate)?

Spill clean-up materials available and laboratory staff are familiar with their use?

Safety shower and eyewash station accessible within 10 seconds?

Safety shower tested and documented within past year?

Eyewash tested, flushed and documented at least monthly?

Fire alarm pull stations, strobes, speakers, and fire extinguisher unobstructed and visible?

Exits clearly marked and unobstructed?

Personal Protection Clothing, Equipment and Engineering Controls

Personnel wear shoes that fully cover feet and full length clothing to protect legs?

Long hair, jewelry, lanyards and other loose articles are confined or removed?

Lab coats of appropriate material available and worn?

Appropriate gloves available and worn?

Appropriate types of eye and face protection (e.g., goggles, face shields, etc.) available and worn?

Appropriate respirators available and used in the laboratory?

Respirator training, fit testing and medical evaluation completed for respirator users?

Respirators cleaned, stored and inspected regularly?

Chemical hood available?

Chemical hood free of clutter?

Chemical hood inspected within last 12 months and capable of drawing at least 100 lfpm (or more or less, as designed)?

Flow indicator is present and working?

Perchloric acid operations conducted in special wash-down hoods?

Biological safety cabinet available?

Biological safety cabinet free of clutter and surfaces are decontaminated?

Biological safety cabinet certified within last 12 months?

Mechanical pipetting used, no mouth suction?

Chemical Safety

Are chemicals used in this area?

Appropriate labels are found on all containers of hazardous chemicals?

Containers are in good conditions (e.g., labels intact, metal containers free of rust) are closed when not in use?

Containers properly segregated by hazard class (e.g., flammables away from oxidizers, acids separate from bases, incompatible acids separated)?

Storage of chemicals above eye level is avoided?

Flammable liquids stored in OSHA/NFPA approved cabinets and safety containers?

Flammable liquids requiring refrigeration stored in either explosion-proof or flammable-resisitant refrigerators and freezers (e.g., no regular refrigerators)?

Ignition sources avoided when using/storing flammables?

Corrosives stored in acid cabinets or other appropriate cabinets?

Peroxide-formers properly labeled and inventory tracked?

Picric acid sufficiently wet?

Large containers (4L or greater) stored near the floor?

Bottle carriers or carts utilized when transporting hazardous chemicals between work areas?

High hazard chemicals used or stored in lab?

Proper signs delineate designated areas where high hazard chemicals are used?

Designated area properly cleaned and decontaminated?

Biological Safety

Are biological materials used in this area?

Biological materials are not stored in hallways in unlocked freezers or refrigerators.

Biohazard signs are posted in labs handling infectious materials (BSL2 and higher).

Disinfectants are on hand for sanitizing bench tops and treating spills.

Biological safety cabinets are available as needed?

Radiation Safety

Are radioactive materials used in this area?

Are meters calibrated?

Are all spaces and items used in radioactive materials research labeled correctly?

Is the radioactive material storage refrigerator or freezer properly labeled?

Are vials of radioactive materials within a refrigerator or freezer stored in a proper "lock box"?

Compressed and Cryogenic Gas Safety

Are compressed gas cylinders used in this area?

Cylinders stored upright and properly secured at all times?

Cylinder caps properly secured when cylinders are not in use?

Regulators always used, proper regulators used for the gas type, pressure bled when not in use?

Cylinders in good condition and clearly marked?

Flammables stored separately from oxidizers, toxics in secure area, etc.?

Cylinders of flammable gases stored in ventilated enclosures?

Cylinders moved on cylinder trucks with regulators removed and caps secured?

Cylinders of gases with NFPA health hazard rating of 3 or 4 with no physiological warning properties are stored in continuously ventilated enclosures?

Are cryogenics gases or liquids used in this area?

Cryogenic gas pressure relief valves in proper working condition?

Face shields and cryo gloves available for transferring cryogenic liquids?

Oxygen monitor available in areas with increased likelihood of oxygen-deficient atmosphere?

Equipment and Physical Hazards Safety

Are equipment safety signs posted and in good condition?

Are all guards and shields in place and secured?

Are safe work practices (long hair tied back, no loose clothing, etc.) being adhered to by all equipment users?

Is equipment in good repair with evidence of proper maintenance?

Are electrical cords in good condition, out of travel paths, and free of any cracks or breaks in insulation?

Is proper PPE available and in use by equipment operators?

Is a tagging system in place to prevent use of damaged equipment?

Is access to equipment restricted?

Have all users been trained to operate this equipment?

Are any additional or new hazards present at or around the equipment?

Have the new or additional hazards been evaluated and documented and have users been made aware of them?

Have there been modifications to the equipment?

Has the equipment been evaluated to determine if new or additional hazards are present?

General Laboratory Safety

Eating, drinking and smoking prohibited in lab?

Lab is maintained secure; door is locked when no one is in lab?

Area conforms to definition of a laboratory?

Appropriate warning signs posted near lab entrance?

Unobstructed aisles maintained at least 36 inches wide throughout area?

Lab benches and work areas free of clutter?

Shelves and cabinets in good condition?

Shelves have seismic restraints, e.g., lips or wires?

Shelves and cabinets secured to walls?

Storage above eye level minimized and items restrained from falling?

Refrigerators and freezers clearly labeled "Not for storage of food for human consumption"?

No storage of food or drink in refrigerators, unless dedicated for such and clearly labeled?

Waste Management

Wastes are not discarded via trash or drain disposal unless specifically approved by the appropriate institutional authority (e.g., Environmental Health and Safety)?

Chemical inventory management/ordering system in place and checked before ordering new chemicals?

Is hazardous chemical waste generated in this area?

Satellite accumulation area near point of generation?

Waste containers tightly closed unless actively adding or removing waste?

Maximum satellite storage area capacity not exceeded (e.g., 55 gallons per hazardous waste stream or 1 quart of acutely hazardous waste)?

Waste container is marked with the words "Hazardous Waste"?

Waste containers is marked with full chemical names identifying the contents stored inside (no abbreviations or formulas)?

Waste containers kept closed except when adding waste?

Waste containers storing liquid hazardous waste at or near sinks and drains are stored within secondary containers?

Secondary containment is in good condition (e.g., free of cracks, gaps and impervious to leaks)?

Is sharps waste (e.g., needles, syringes, scalpel blades, or other instruments that have the potential to cut, puncture, or abrade skin) generated in this area?

Sharps wastes are immediately discarded into proper puncture-resistant containers?

Sharps containers are readily available and managed appropriately (e.g., not overfilled)?

Is biological waste generated in this area?

Biological waste liquids are decontaminated (if applicable) prior to drain disposal?

Biological solids discarded as regulated medical waste and autoclaved or disinfected as appropriate?

Is radioactive waste generated in this area?

Is mixed waste (e.g., scintillation vials and any other radioactive and hazardous chemical waste mixture) generated in this area and properly managed?

Are the radioactive waste containers properly labeled?

Additional Comments

Additional comments/observations:

American Chemical Society (ACS) Lab Safety Checklist

Created by: American Chemical Socieity | Industry: Education | Downloads: 92

General Lab Safety Checklist

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Audit

Training and Documentation

Up-to-date inventory maintained for all hazardous materials?

Chemical Safety Data Sheets (SDS) maintained and readily available at all times employees are present?

Workplace hazard assessment and certification completed?

Lab workers received institutional safety training (typically provided by Environmental Health and Safety office) and supplemental laboratory-specific safety training for the hazards present in the laboratory?

Lab workers are familiar with physical and health hazards of chemicals in work area?

Lab workers able to describe how to detect the presence or release of hazardous materials?

Lab workers know how to protect themselves and others from effects of hazardous materials?

Chemical hygiene plan readily available and laboratory workers are familiar with it?

Spill and Emergency Planning

Lab workers familiar with the fire safety and building evacuation procedures including evacuation routes, nearest fire exits, fire alarm pull stations, and fire extinguishers?

Emergency procedures and phone numbers clearly posted?

First aid materials readily available and up-to-date?

Required "antidotes" or special first aid materials available (e.g., hydrofluoric acid requires calcium gluconate)?

Spill clean-up materials available and laboratory staff are familiar with their use?

Safety shower and eyewash station accessible within 10 seconds?

Safety shower tested and documented within past year?

Eyewash tested, flushed and documented at least monthly?

Fire alarm pull stations, strobes, speakers, and fire extinguisher unobstructed and visible?

Exits clearly marked and unobstructed?

Personal Protection Clothing, Equipment and Engineering Controls

Personnel wear shoes that fully cover feet and full length clothing to protect legs?

Long hair, jewelry, lanyards and other loose articles are confined or removed?

Lab coats of appropriate material available and worn?

Appropriate gloves available and worn?

Appropriate types of eye and face protection (e.g., goggles, face shields, etc.) available and worn?

Appropriate respirators available and used in the laboratory?

Respirator training, fit testing and medical evaluation completed for respirator users?

Respirators cleaned, stored and inspected regularly?

Chemical hood available?

Chemical hood free of clutter?

Chemical hood inspected within last 12 months and capable of drawing at least 100 lfpm (or more or less, as designed)?

Flow indicator is present and working?

Perchloric acid operations conducted in special wash-down hoods?

Biological safety cabinet available?

Biological safety cabinet free of clutter and surfaces are decontaminated?

Biological safety cabinet certified within last 12 months?

Mechanical pipetting used, no mouth suction?

Chemical Safety

Are chemicals used in this area?

Appropriate labels are found on all containers of hazardous chemicals?

Containers are in good conditions (e.g., labels intact, metal containers free of rust) are closed when not in use?

Containers properly segregated by hazard class (e.g., flammables away from oxidizers, acids separate from bases, incompatible acids separated)?

Storage of chemicals above eye level is avoided?

Flammable liquids stored in OSHA/NFPA approved cabinets and safety containers?

Flammable liquids requiring refrigeration stored in either explosion-proof or flammable-resisitant refrigerators and freezers (e.g., no regular refrigerators)?

Ignition sources avoided when using/storing flammables?

Corrosives stored in acid cabinets or other appropriate cabinets?

Peroxide-formers properly labeled and inventory tracked?

Picric acid sufficiently wet?

Large containers (4L or greater) stored near the floor?

Bottle carriers or carts utilized when transporting hazardous chemicals between work areas?

High hazard chemicals used or stored in lab?

Proper signs delineate designated areas where high hazard chemicals are used?

Designated area properly cleaned and decontaminated?

Biological Safety

Are biological materials used in this area?

Biological materials are not stored in hallways in unlocked freezers or refrigerators.

Biohazard signs are posted in labs handling infectious materials (BSL2 and higher).

Disinfectants are on hand for sanitizing bench tops and treating spills.

Biological safety cabinets are available as needed?

Radiation Safety

Are radioactive materials used in this area?

Are meters calibrated?

Are all spaces and items used in radioactive materials research labeled correctly?

Is the radioactive material storage refrigerator or freezer properly labeled?

Are vials of radioactive materials within a refrigerator or freezer stored in a proper "lock box"?

Compressed and Cryogenic Gas Safety

Are compressed gas cylinders used in this area?

Cylinders stored upright and properly secured at all times?

Cylinder caps properly secured when cylinders are not in use?

Regulators always used, proper regulators used for the gas type, pressure bled when not in use?

Cylinders in good condition and clearly marked?

Flammables stored separately from oxidizers, toxics in secure area, etc.?

Cylinders of flammable gases stored in ventilated enclosures?

Cylinders moved on cylinder trucks with regulators removed and caps secured?

Cylinders of gases with NFPA health hazard rating of 3 or 4 with no physiological warning properties are stored in continuously ventilated enclosures?

Are cryogenics gases or liquids used in this area?

Cryogenic gas pressure relief valves in proper working condition?

Face shields and cryo gloves available for transferring cryogenic liquids?

Oxygen monitor available in areas with increased likelihood of oxygen-deficient atmosphere?

Equipment and Physical Hazards Safety

Are equipment safety signs posted and in good condition?

Are all guards and shields in place and secured?

Are safe work practices (long hair tied back, no loose clothing, etc.) being adhered to by all equipment users?

Is equipment in good repair with evidence of proper maintenance?

Are electrical cords in good condition, out of travel paths, and free of any cracks or breaks in insulation?

Is proper PPE available and in use by equipment operators?

Is a tagging system in place to prevent use of damaged equipment?

Is access to equipment restricted?

Have all users been trained to operate this equipment?

Are any additional or new hazards present at or around the equipment?

Have the new or additional hazards been evaluated and documented and have users been made aware of them?

Have there been modifications to the equipment?

Has the equipment been evaluated to determine if new or additional hazards are present?

General Laboratory Safety

Eating, drinking and smoking prohibited in lab?

Lab is maintained secure; door is locked when no one is in lab?

Area conforms to definition of a laboratory?

Appropriate warning signs posted near lab entrance?

Unobstructed aisles maintained at least 36 inches wide throughout area?

Lab benches and work areas free of clutter?

Shelves and cabinets in good condition?

Shelves have seismic restraints, e.g., lips or wires?

Shelves and cabinets secured to walls?

Storage above eye level minimized and items restrained from falling?

Refrigerators and freezers clearly labeled "Not for storage of food for human consumption"?

No storage of food or drink in refrigerators, unless dedicated for such and clearly labeled?

Waste Management

Wastes are not discarded via trash or drain disposal unless specifically approved by the appropriate institutional authority (e.g., Environmental Health and Safety)?

Chemical inventory management/ordering system in place and checked before ordering new chemicals?

Is hazardous chemical waste generated in this area?

Satellite accumulation area near point of generation?

Waste containers tightly closed unless actively adding or removing waste?

Maximum satellite storage area capacity not exceeded (e.g., 55 gallons per hazardous waste stream or 1 quart of acutely hazardous waste)?

Waste container is marked with the words "Hazardous Waste"?

Waste containers is marked with full chemical names identifying the contents stored inside (no abbreviations or formulas)?

Waste containers kept closed except when adding waste?

Waste containers storing liquid hazardous waste at or near sinks and drains are stored within secondary containers?

Secondary containment is in good condition (e.g., free of cracks, gaps and impervious to leaks)?

Is sharps waste (e.g., needles, syringes, scalpel blades, or other instruments that have the potential to cut, puncture, or abrade skin) generated in this area?

Sharps wastes are immediately discarded into proper puncture-resistant containers?

Sharps containers are readily available and managed appropriately (e.g., not overfilled)?

Is biological waste generated in this area?

Biological waste liquids are decontaminated (if applicable) prior to drain disposal?

Biological solids discarded as regulated medical waste and autoclaved or disinfected as appropriate?

Is radioactive waste generated in this area?

Is mixed waste (e.g., scintillation vials and any other radioactive and hazardous chemical waste mixture) generated in this area and properly managed?

Are the radioactive waste containers properly labeled?

Additional Comments

Additional comments/observations: