Biohazard/rDNA Use

  • Are Biohazards or rDNA in use?

  • List hazards

  • Are Biohazards/rDNA registered with IBC?

  • Is a biosafety cabinet needed for type of work?

  • Is there a biosafety cabinet in the lab?

  • What type of ventilation does the biosafety cabinet have?

  • Is the biosafety cabinet inspected within 12 months?

  • Is PPE recommended or required?

  • List the necessary PPE

  • Is PPE worn by staff for biohazard/rDNA work?

  • Are live animals brought to the lab?

  • Are carcasses stored in the lab?

  • Is disinfectant used in lab?

  • List disinfectant used.

  • Describe disinfectant use.

  • Is there a procedure in place for biohazard spill response?

  • Is the response procedure appropriate?

  • Is the staff familiar with the procedure for biohazard exposure response?

  • When was the most recent bloodborne pathogens training for staff?

  • Is equipment labeled with biohazard tags?

  • Is biohazardous material vacuum trapped?

  • Is there disinfectant in the trap?

  • Is biohazardous material filtered?

Biosafety Level 2 Agent Work

  • Is there any biosafety level 2 agent work conducted in the lab?

  • Is a hand washing sink available near the exit door?

  • Is food for human consumption stored in the lab?

  • Are mechanical pipetting devices used?

  • Are sharps used?

  • Are needles recapped?

  • Are sharps disposed of in sharps containers?

  • Is plasticware substituted for glassware where possible?

  • Are BL2 agents worked with on an open bench?

  • List precautions followed

Infectious Waste Disposal

  • Is there evidence of infectious waste disposal?

  • Select types of infectious waste being disposed.

  • Is rDNA or biohazard waste recommended to be autoclaved?

  • Is an autoclave available?

  • Is there verification of the autoclave effectiveness.

  • Are materials to be autoclaved transported in leakproof containers?

  • Are work surfaces cleanable and non-porous

  • Is a centrifuge in use?

  • Is a lab-specific biosafety manual available?

  • Is in-lab biosafety training performed?

  • Are protective lab coats worn?

  • Are lab coats removed upon leaving lab?

  • Are lab coats laundered?

  • How are they laundered?

  • Is eye protection worn in the lab?

  • When is eye protection worn?

Infectious Waste

  • Are sharps used in the lab?

  • Are there sharps boxes for disposal?

  • Is infectious fluid waste generated?

  • Is there plastic debris contaminated with infectious materials?

  • Is an autoclave available?

  • Is infectious waste autoclaved?

  • Is there verification of the autoclave effectiveness?

  • Are materials to be autoclaved transported in leak proof containers?

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