Biosafety Audit - Princeton University EHS rev 09092013

• Principal Investigator/ Room Number

• Department

• Building

• Conducted on

  Date

• Prepared by

Biohazard/rDNA Use

• Are Biohazards or rDNA in use?

  Yes

  No

  N/A

• List hazards

  Yes

  No

  N/A

• Are Biohazards/rDNA registered with IBC?

  Yes

  No

  N/A

• Is a biosafety cabinet needed for type of work?

  Yes

  No

  N/A

• Is there a biosafety cabinet in the lab?

  Yes

  No

  N/A

• What type of ventilation does the biosafety cabinet have?

  Yes

  No

  N/A

• Is the biosafety cabinet inspected within 12 months?

  Yes

  No

  N/A

• Is PPE recommended or required?

  Yes

  No

  N/A

• List the necessary PPE

  Yes

  No

  N/A

• Is PPE worn by staff for biohazard/rDNA work?

  Yes

  No

  N/A

• Are live animals brought to the lab?

  Yes

  No

  N/A

• Are carcasses stored in the lab?

  Yes

  No

  N/A

• Is disinfectant used in lab?

  Yes

  No

  N/A

• List disinfectant used.

  Yes

  No

  N/A

• Describe disinfectant use.

  Yes

  No

  N/A

• Is there a procedure in place for biohazard spill response?

  Yes

  No

  N/A

• Is the response procedure appropriate?

  Yes

  No

  N/A

• Is the staff familiar with the procedure for biohazard exposure response?

  Yes

  No

  N/A

• When was the most recent bloodborne pathogens training for staff?

  Yes

  No

  N/A

• Is equipment labeled with biohazard tags?

  Yes

  No

  N/A

• Is biohazardous material vacuum trapped?

  Yes

  No

  N/A

• Is there disinfectant in the trap?

  Yes

  No

  N/A

• Is biohazardous material filtered?

  Yes

  No

  N/A

Biosafety Level 2 Agent Work

• Is there any biosafety level 2 agent work conducted in the lab?

  Yes

  No

  N/A

• Is a hand washing sink available near the exit door?

  Yes

  No

  N/A

• Is food for human consumption stored in the lab?

  Yes

  No

  N/A

• Are mechanical pipetting devices used?

  Yes

  No

  N/A

• Are sharps used?

  Yes

  No

  N/A

• Are needles recapped?

  Yes

  No

  N/A

• Are sharps disposed of in sharps containers?

  Yes

  No

  N/A

• Is plasticware substituted for glassware where possible?

  Yes

  No

  N/A

• Are BL2 agents worked with on an open bench?

  Yes

  No

  N/A

• List precautions followed

  Yes

  No

  N/A

Infectious Waste Disposal

• Is there evidence of infectious waste disposal?

  Yes

  No

  N/A

• Select types of infectious waste being disposed.

  Yes

  No

  N/A

• Is rDNA or biohazard waste recommended to be autoclaved?

  Yes

  No

  N/A

• Is an autoclave available?

  Yes

  No

  N/A

• Is there verification of the autoclave effectiveness.

  Yes

  No

  N/A

• Are materials to be autoclaved transported in leakproof containers?

  Yes

  No

  N/A

• Are work surfaces cleanable and non-porous

  Yes

  No

  N/A

• Is a centrifuge in use?

  Yes

  No

  N/A

• Is a lab-specific biosafety manual available?

  Yes

  No

  N/A

• Is in-lab biosafety training performed?

  Yes

  No

  N/A

• Are protective lab coats worn?

  Yes

  No

  N/A

• Are lab coats removed upon leaving lab?

  Yes

  No

  N/A

• Are lab coats laundered?

  Yes

  No

  N/A

• How are they laundered?

  Yes

  No

  N/A

• Is eye protection worn in the lab?

  Yes

  No

  N/A

• When is eye protection worn?

  Yes

  No

  N/A

Infectious Waste

• Are sharps used in the lab?

  Yes

  No

  N/A

• Are there sharps boxes for disposal?

  Yes

  No

  N/A

• Is infectious fluid waste generated?

  Yes

  No

  N/A

• Is there plastic debris contaminated with infectious materials?

  Yes

  No

  N/A

• Is an autoclave available?

  Yes

  No

  N/A

• Is infectious waste autoclaved?

  Yes

  No

  N/A

• Is there verification of the autoclave effectiveness?

  Yes

  No

  N/A

• Are materials to be autoclaved transported in leak proof containers?

  Yes

  No

  N/A