Audit

BSC & Buffer Prep area
Sort

All unnecessary equipment, furniture, Storage (racks pipette holder etc.) have been removed

All unfinished work has been moved away or is clearly identified

All unnecessary tools, paperwork, supplies (pipette tips, wipes, stationary) have been removed.

Supply cupboard is organised and tidy. Powders on floor are organised and tidy. Bench is organised and Tidy

reagent and chemical cabinets are organised and tidy

Balance hood is organised and tidy

Straighten

Location of bins, consumables, PPE, parts are clearly defined by lines and labels

Balances are placed in correct location

Tools, Paperwork, Office Supplies are labeled and have a defined location that is visible

Floor markings are clearly identified

Shine

Used pipette and GMM bins are at an acceptable level (and not overflowing)

Work benches are clean and free of debris (including walls and sides)

Inside balance area is clean, including sides and free of debris

Balances and printers are clean and free of debris

Tools are clean and neatly returned to designated areas

Floors are free from dirt, debris, spills, parts, etc.

Standardise

Stock cupboard is stocked to appropriate levels (Pippettes, falcon tubes. filters)

BSC PPE is stocked

ViCELL reagent pack Kanban is being being followed

IPA Kanban is being followed

PM/ calibration stickers are in place, in date and are clearly visible (balance, Balance hood, BSC)

Sustain

It is evident that the area owner is involved in 6S activities and improvement initiatives

Overall, the principles of 6S look to be understood in the area

Safety

Is all BSC PPE readily available

Are emergency procedures and equipment clearly visible/ accessible

Are doorways and walkways kept clear and free from obstruction

Fume hood, back sink, buffer rack
Sort

All unnecessary equipment, furniture, Storage (racks pipette holder etc.) have been removed

All unfinished work has been moved away or is clearly identified

All unnecessary tools, paperwork, supplies (pipette tips, wipes, stationary) have been removed.

UFDF Cupboard and shelf is organised and tidy

Sink (including underneath) is organised and tidy

Fume hood is organised and tidy

Buffer rack is organised and tidy

Straighten

Equipment in fume hood (including racks) is identified and placed in correct location

Shine

UFDF bench is clean and free of debris (including splash guards, back of fume hood and under sink)

Fume hood is clean and free from splashes

Equipment in fume hood is clean and free from splashes

Sink area is all clean and free of debris (including splash guards and under sink)

Floors are free from dirt, debris, spills, parts, etc

Standardise

In date PM/ calibration stickers are in place for pH and Conductivity meter and fume hood

Buffers on buffer rack are all labeled and within date

Visual and written aids are used within the area (photos, checklists, diagrams)

Sustain

It is evident that the area owner is involved in 6S activities and improvement initiatives

Overall, the principles of 6S look to be understood in the area

Safety

All PPE (lab apron, green gloves, visor) are all available/accessible

Are emergency procedures and equipment clearly visible/accessible

Are doorways and walkways kept clear and free from obstruction

AKTAs
Sort

All unnecessary equipment, furniture, Storage (racks, pipette holder etc.) have been removed

All unfinished work has been removed or is clearly identified

All unnecessary tools, paperwork, supplies (pipette tips, wipes, stationary) have been removed.

AKTA drawers are organised and Tidy

AKTA cupboards are organised and tidy

Straighten

Tools are placed in defined location

Consumables are placed in defined location (pipette tips, wipes, stationary)

AKTA computer key boards and mice are placed neatly on bench

Shine

GMM bin is at an acceptable level (not overflowing)

work benches are clean and free of debris

AKTA's are clean and free from debris (including sides and underneath)

Tools are clean and neatly returned to designated areas

Floor is free from dirt, debris, spills, parts, etc.

Standardise

AKTA PM/ calibration stickers are in place, in date and are clearly visible

Gloves are stocked and have correct sizes available

Sustain

It is evident that the area owner is involved in 6S activities and improvement initiatives

Overall, the principles of 6S look to be understood in the area

Safety

Is all necessary PPE available

Are emergency procedures and equipment clearly visible/accessible

Are doorways and walkways kept clear and free from obstruction

HPLC, UPLC, DASGIPs
Sort

All unnecessary equipment, furniture, Storage (racks pipette holder etc.) have been removed

All unfinished work has been removed or is clearly identified

All unnecessary tools, paperwork, supplies (pipette tips, wipes, stationary) have been removed.

UPLC cupboard is organised and tidy

Cupboard under Dasgips is organised and tidy

Drawers are organised and tidy

Straighten

GMM bin, UPLC consumables, PPE, clearly defined by lines and labels

HPLC, UPLC, Dasgip computer and Dasgips are identified and placed in a defined location

Floor markings are clearly identified

Shine

Waste in GMM bin is at an acceptable level

Waste levels in sharps bin is at an acceptable level

HPLC/UPLC waste duran is at an acceptable level

Work surfaces/ Equipment are clean and free of debris and splashes (including walls)

Tools are clean and neatly returned to designated areas

Floors are free from dirt, debris, spills, parts, etc.

Standardise

Leur lock syringes and alcohol wipes are stocked

HPLC consumables are stocked

HPLC, UPLC and Dasgip PM/Calibration stickers are visable and in date

Sustain

It is evident that the area owner is involved in 6S activities and improvement initiatives

Overall, the principles of 6S look to be understood in the area

Safety

Gloves are available (and appropriate sizes are also available)

Are emergency procedures and equipment clearly visible/ accessible

Are doorways and walkways kept clear and free from obstruction

Glassware, SoloVPE, Printers
Sort

All unnecessary equipment, furniture, Storage, paperwork has been removed

All unfinished work has been removed or is clearly identified

All unnecessary paper/printer consumables have been removed

Straighten

Printer shelves are organised and tidy

Glassware cabinet is organised with glassware in its designated area

Solovp cupboard is organised and tidy

Shine

Solovp is clean and free from spills/splashes

Bench is clean and free of debris

Waste levels in sharps bin is at an acceptable level

Standardise

SoloVP consumables are stocked and readily available

All printers are working and ready to use (with correct label size)

PM/ calibration stickers are in place, in date and are clearly visible

Sustain

It is evident that the area owner is involved in 6S activities and improvement initiatives

Overall, the principles of 6S look to be understood in the area

Safety

Is there sufficient amount of spill mat available

Are emergency procedures and equipment clearly visible/ accessible

Are doorways and walkways kept clear and free from obstruction

Bioreactor build area
Sort

All unnecessary equipment, furniture, Storage (racks pipette holder etc.) have been removed

All unfinished work has been removed or is clearly identified

All unnecessary tools, paperwork, supplies (pipette tips, wipes, stationary) have been removed.

Cupboards are organised and tidy

Drawers are organised and tidy

Straighten

Tools are returned to holder and are organised

Lab folders are organised and tidy

Bioreactor components are placed in correct drawers

Shine

Benches are clean and free from debris

Tools are clean

Floors are free from dirt, debris, spills, parts, etc.

Standardise

Reactor tubing is stocked

Tie wraps are stocked

Autoclave bags stocked

Sustain

It is evident that the area owner is involved in 6S activities and improvement initiatives

Overall, the principles of 6S look to be understood in the area

Safety

Tubing grip gloves are available

Are emergency procedures and equipment clearly visible/ accessible

Are doorways and walkways kept clear and free from obstruction

5L Bioreactor area
Sort

All unnecessary equipment, furniture, Storage (racks pipette holder etc.) has been removed

All unfinished work has been removed or is clearly identified

All unnecessary tools, paperwork, supplies (syringes, IPA wipes etc.) have been removed.

Drawers are organised

Cupboards are organised

Straighten

GMM liquid and GMM waste bins, PPE and sampling consumables are clearly defined by lines and labels

Equipment, Machines, Furniture, Racking is identified and placed in a defined location

Tools, Paperwork, Office Supplies are labeled and have a defined location that is visible

Floor markings are clearly identified

Shine

GMM bins are at an acceptable level

Work surfaces/ Equipment are clean and free of debris and splashes (including walls)

Tools are clean and neatly returned to designated areas

Floors are free from dirt, debris, spills, parts, etc.

Standardise

Drawers are stocked (sample lines, syringes, alcohol wipes and pump tubing)

Leur lock syringes and Alcohol wipes stocked

PM/ calibration stickers are in place, in date and are clearly visible

Visual and written aids are used within the area (photos, checklists, diagrams)

Sustain

It is evident that the area owner is involved in 6S activities and improvement initiatives

Overall, the principles of 6S look to be understood in the area

Safety

Is all appropriate PPE available (gloves)

Are emergency procedures and equipment clearly visible/ accessible

Are doorways and walkways kept clear and free from obstruction

10L Bioreactor area
Sort

All unnecessary equipment, furniture, Storage (racks pipette holder etc.) has been removed

All unfinished work has been removed or is clearly identified

All unnecessary tools, paperwork, supplies (syringes, IPA wipes etc.) have been removed.

Cupboards are organised and tidy

Perfusion equipment is neatly stored under benches

Straighten

Equipment, Machines, Furniture, Racking is identified and placed in a defined location

Tools, Paperwork, Office Supplies are labeled and have a defined location that is visible

Shine

Work surfaces/ Equipment are clean and free of debris and splashes (including walls)

Tools are clean and neatly returned to designated areas

Floors are free from dirt, debris, spills, parts, etc.

Is Hazardous bin waste at an acceptable level

Standardise

PM/ calibration stickers are in place, in date and are clearly visible

Sustain

It is evident that the area owner is involved in 6S activities and improvement initiatives

Overall, the principles of 6S look to be understood in the area

Safety

Is all appropriate PPE available (gloves)

Are emergency procedures and equipment clearly visible/ accessible

Are doorways and walkways kept clear and free from obstruction

Preculture and centrifuge
Sort

All unnecessary equipment, furniture, Storage (racks pipette holder etc.) has been removed

All unfinished work has been removed or is clearly identified

All unnecessary tools, paperwork, supplies (syringes, IPA wipes etc.) have been removed.

Cupboards are organised and tidy

Straighten

Osmo consumables are in the right place

Equipment, Machines, Furniture, Racking is identified and placed in a defined location

Tools, Paperwork, Office Supplies are labeled and have a defined location that is visible

Bench and shelf is clean and free of debris

Tools are clean and neatly returned to designated areas

Floors are free from dirt, debris, spills, parts, etc.

Osmo sharps bin is at an acceptable level

PM/ calibration stickers are in place, in date and are clearly visible

Standardise

Shake flasks are stocked

PM/calibration stickers are in place and visible (Waverockers, GE wave rockers, Ambr, water bath, cO2 incubators, Centrifuges, Osmo)

Sustain

It is evident that the area owner is involved in 6S activities and improvement initiatives

Overall, the principles of 6S look to be understood in the area

Safety

Is all appropriate PPE available (gloves)

Are emergency procedures and equipment clearly visible/ accessible

Are doorways and walkways kept clear and free from obstruction

Cold room
Sort

All unnecessary trolleys have been removed

All old/ unwanted samples have been removed

All samples are organised and tidy

Liquid medias are organised and tidy

Powders are organised and tidy

Straighten

all sample are placed in designated area

Shine

shelves are clean and free of debris

Floors are free from dirt, debris, spills, parts, etc.

Medias, powders, walls and shelves are free from mold

Mold present

Sustain

It is evident that the area owner is involved in 6S activities and improvement initiatives

Overall, the principles of 6S look to be understood in the area

Safety

Are emergency procedures and equipment clearly visible/ accessible

Are doorways and walkways kept clear and free from obstruction

IPT Room
Sort

All unnecessary equipment, furniture, Storage (racks pipette holder etc.) has been removed

All unfinished work has been removed or is clearly identified

All unnecessary tools, paperwork, supplies (syringes, IPA wipes etc.) have been removed.

Cupboards under bench are organised and tidy

cabinet is organised and tidy

Drawers are organised and tidy

Straighten

CEDEX cups, ViCell cups and pipette tips have a defined location

Equipment, Machines, Furniture, Racking is identified and placed in a defined location

Tools, Paperwork, Office Supplies are labeled and have a defined location that is visible

Shine

Bench is clean and free from debris

Tools are clean and neatly returned to designated areas

Floors are free from dirt, debris, spills, parts, etc.

GMM bin is at an acceptable level

Pipette tip bin is at an acceptable level

Standardise

CEDEX cups, ViCELL cups and pipette tips are stocked and available

50ml and 15ml falcon tubes are stocked

Back up CEDEX and ViCELL consumables stocked in cupboards

bin bags stocked

PM/calibration stickers are in place and visible (Waverockers, GE wave rockers, Ambr, water bath, cO2 incubators, Centrifuges, Osmo)

Sustain

It is evident that the area owner is involved in 6S activities and improvement initiatives

Overall, the principles of 6S look to be understood in the area

Safety

Is all appropriate PPE available (gloves)

Are emergency procedures and equipment clearly visible/ accessible

Are doorways and walkways kept clear and free from obstruction

Stockroom
Sort

All unnecessary equipment, furniture, Storage (racks pipette holder etc.) has been removed

All unnecessary tools, paperwork, supplies have been removed.

Cupboards are organised and tidy

Cupboards are organised and tidy

Shelves are organised and tidy

Straighten

Osmo consumables are organised and tidy

Equipment, Machines, Furniture, Racking is identified and placed in a defined location

Tools, Paperwork, Office Supplies are labeled and have a defined location that is visible

Shine

Cupboards and shelves are clean and free from debris

Floors are free from dirt, debris, spills, parts, etc.

Safety Shoes neatly stored in shelving

Standardise

Stocks levels are being managed correctly

Lab stock is within date (stock rotation happening)

Sustain

It is evident that the area owner is involved in 6S activities and improvement initiatives

Overall, the principles of 6S look to be understood in the area

Safety

Are emergency procedures and equipment clearly visible/ accessible

Are doorways and walkways kept clear and free from obstruction

Please note that this checklist is a hypothetical example and provides basic information only. It is not intended to take the place of, among other things, workplace, health and safety advice; medical advice, diagnosis, or treatment; or other applicable laws. You should also seek your own professional advice to determine if the use of such checklist is permissible in your workplace or jurisdiction.