Containment Level 2 Biosafety Inspection checklist (CBS3-2023)

• Site conducted

  Select

• Conducted on

  Date

• Prepared by

• Location(s)

• Biosafety Permit Number

• Responsible Person

• Personnel Present

Signage

• 1. Door Sign for Hazardous Materials Posted with emergency contacts (CBS 3.2.2)

  Yes

  No

  N/A

• 1.1. Door Hazard Sign is Up-To-Date

  Yes

  No

  N/A

• 2. Biosafety Permit Posted (SFU)

  Yes

  No

  N/A

• 2.1. Biosafety Permit is current

  Yes

  No

  N/A

• 3. International Biohazard Symbol Posted (CBS3 3.2.2)

  Yes

  No

  N/A

• 4. Entrance Requirements Posted (CBS3 3.2.2)

  Yes

  No

  N/A

• Photo(s)

  Media

Physical requirements

• 5. Lab Has a Door (CBS3 3.2.4)

  Yes

  No

  N/A

• 5.1. Door is kept closed

  Yes

  No

  N/A

• 5.2. Door is lockable

  Yes

  No

  N/A

• 5.2.1. Door is kept locked

  Yes

  No

  N/A

• 5.3. If not, access is otherwise restricted

  Yes

  No

  N/A

• 6. Lab Has Windows (CBS3 3.1.1)

  Yes

  No

  N/A

• 6.1. Windows Open (CBS3 3.1.1)

  Yes

  No

  N/A

• 6.1.1. Windows Have Screens (CBS3 3.1.1)

  Yes

  No

  N/A

• 6.1.1.1. SOP - Windows not to be Opened (CBS3 3.1.1)

  Yes

  No

  N/A

• 7. PPE Storage Space (CBS3 3.2.9))

  Yes

  No

  N/A

• 8. Personal clothing and belongings stored way from PPE. (CBS3 4.4.8, 4.4.9)

  Yes

  No

  N/A

• 9. Sink for Hand Washing available in the lab (CBS3 3.5.4)

  Yes

  No

  N/A

• 10. Eyewash and Shower available inside the containment zone or nearby (WorkSafe BC)

  Yes

  No

  N/A

• 11. Lab clean and free of obstructions, unnecessary materials, and items that cannot be easily decontaminated (CBS3 4.5.3)

  Yes

  No

  N/A

• 12. Surfaces cleanable, non-absorbent, and resistant to physical damage and damage caused by decontamination (Benchetops, Casework, Floors, Chairs) (CBS3 3.3.1)

  Yes

  No

  N/A

• 13. Paperwork/office work in the lab ( CBS3 4.5.2)

  Yes

  No

  N/A

• 13.1. Mechanism in place to separate the paperwork/ office work area from the lab bench to prevent the spread of contamination (CBS3 4.5.2)

  Yes

  No

  N/A

• 14. Biosafety Cabinet (BSC) in the lab (CBS3 3.6.1)

  Yes

  No

  N/A

• 14.1. BSc installed at an appropriate location (CBS3 4.5.22)

  Yes

  No

  N/A

• 14.2. BSC Certified? (CBS3 5.1.6)

  Yes

  No

  N/A

• 14.3. Lab members trained on safe work practices with BSC (CBS3 4.2.2)

  Yes

  No

  N/A

• 14.3.1. Normal working conditions of BSC verified before use (CBS3 4.5.9))

  Yes

  No

  N/A

• 15. Other primary containment devices or ventilated devices (CBS3 3.6.2, CBS 5.1.8)

  Yes

  No

  N/A

• 15.1. Designed to prevent the release of biohazards or have a mechanism in place to contain a potential release (CBS3 3.6.2)

  Yes

  No

  N/A

• 15.2. Routinely verified and tested (according to an accepted SOP) (CBS3 4.5.9, CBS3 5.1.6)

  Yes

  No

  N/A

• 15.3. SOP to transfer biohazards from one to another without aerosols/release (closed systems) (CBS3 4.5.19)

  Yes

  No

  N/A

• 16. Does the lab have its OWN autoclave? (CBS3 3.6.5)

  Yes

  No

  N/A

• 16.1. Following Biohazard Transport SOP to transport waste to the autoclave room (CBS3 3.6.5, CBS3 4.7.7)

  Yes

  No

  N/A

• 16.1.1. Lab Specific SOP (CBS3 3.6.5, CBS3 4.7.7)

  Yes

  No

  N/A

• 16.2. Parameters monitored and recorded?

  Yes

  No

  N/A

• 16.3. Validation records? (annual)

  Yes

  No

  N/A

• 17. Biologicals Aspirated or under Vacuum (CBS3 3.6.8)

  Yes

  No

  N/A

• 17.1. Vacuum protected by Filter/Trap and/or disinfectant trap (CBS3 3.6.8)

  Yes

  No

  N/A

• 17.1.1. Filter inspected routinely (e.g. monthly), replaced regularly (e.g. annually) and documented (CBS3 4.5.8)

  Yes

  No

  N/A

• 18. Centrifuges

  Yes

  No

  N/A

• 18.1. Centrifuges have sealed rotors/cups (CBS3 4.5.23)

  Yes

  No

  N/A

• 18.1.1. Inhalation is the primary route of infection for agents centrifuged Centrifuges have sealed rotors/cups (CBS3 4.5.23)

  Yes

  No

  N/A

• 19. Is there a telephone in the lab?

  Yes

  No

  N/A

• 19.1. Other 2-way communication from Lab?

  Yes

  No

  N/A

• 20. Biological material stored outside the containment zone.

  Yes

  No

  N/A

• 20.1. Containers labelled, leakproof, impact resistant (CBS3 4.5.14)

  Yes

  No

  N/A

• 20.2. In locked storage equipment or within an area with limited access. (CBS3 4.5.14) with biohazard signage, risk group, and emergency signage posted at the point of entry or on the equipment (CBS3 3.2.3)

  Yes

  No

  N/A

PPE

• 21. Closed Shoes

  Yes

  No

  N/A

• 22. Covered legs

  Yes

  No

  N/A

• 23. Lab coat

  Yes

  No

  N/A

• 24. PPE is specific to each Containment Zone (CBS3 4.4.13) and donned appropriately

  Yes

  No

  N/A

• 25. Gloves for biohazards (CBS3 4.3.3)

  Yes

  No

  N/A

• 25.1. Gloves removed and hands washed before exiting (CBS3 4.4.19)

  Yes

  No

  N/A

• 26. Face protection for splashes or flying objects (CBS3 4.4.13)

  Yes

  No

  N/A

• 27. Long hair is tied up (CBS3 4.5.5) and jewelry that may become contaminated or compromise PPE must be removed or covered (CBS3 4.4.12)

  Yes

  No

  N/A

• 28. Respirators Used

  Yes

  No

  N/A

• 28.1. Respiratory protection program in place (WorkSafe BC)

  Yes

  No

  N/A

• 29. PPE is to be doffed in a manner that minimizes contamination of the skin and hair (CBS3 4.4.18)

  Yes

  No

  N/A

Practices

• 30. New Staff Receive Lab-Specific Orientation and Training

  Yes

  No

  N/A

• 30.1. Includes physical design and operation of the containment zone and containment systems (CBS3 4.2.2)

  Yes

  No

  N/A

• 30.2. Includes use and operation of laboratory equipment, including primary containment devices (CBS3 4.2.2)

  Yes

  No

  N/A

• 30.3. Includes exposure control (CBS3 4.2.2)

  Yes

  No

  N/A

• 30.4. Includes signs and symptoms of exposure (CBS3 4.2.2)

  Yes

  No

  N/A

• 30.5. Personnel to demonstrate knowledge of and proficiency in the SOPs on which they were trained (CBS3 4.2.2)

  Yes

  No

  N/A

• 30.6. Trainees directly supervised till all training complete (CBS3 4.2.2)

  Yes

  No

  N/A

• 32. Wounds, Cuts, Scratches Covered with waterproof dressings (CBS3 4.4.11)

  Yes

  No

  N/A

• 58. Lab has an appropriate spill kit & procedure (CBS3 4.8.8)

  Yes

  No

  N/A

• 33. No admin work/laptops (except in designated Paper/Computer work stations) (CBS3 4.5.2)

  Yes

  No

  N/A

• 34. Procedure in place to prevent personal exposure (good microbiological laboratory practices, decontamination of surfaces and items that may be contaminated, and removal of PPE when it may have become contaminated.) (CBS3 4.5.1)

  Yes

  No

  N/A

• 35. Needles/Sharps used

  Yes

  No

  N/A

• 35.1. Are there suitable alternatives? (CBS3 4.5.6)

  Yes

  No

  N/A

• 35.2. Bending, shearing, re-capping, or removing needles from syringes

  Yes

  No

  N/A

• 35.2.1. SOP for doing safely. (CBS3 4.5.7)

  Yes

  No

  N/A

• 35.3. Sharps containers used (CBS3 4.7.4)

  Yes

  No

  N/A

• 36. Work with human tissue/fluids (except cell lines, urine)

  Yes

  No

  N/A

• 36.1. Staff offered Hep B Vaccine and record documented? (CBS3 4.1.9)

  Yes

  No

  N/A

• 37. Lab handling non-human primates?

  Yes

  No

  N/A

• 37.1. Personnel issued Emergency medical contact cards (CBS3 4.8.3)

  Yes

  No

  N/A

• 38. Disinfectant & neutralizing chemicals for toxins available in the lab. (CBS3 4.7.3)

  Yes

  No

  N/A

• 38.1. Disinfectant & neutralizing chemicals for toxins are effective against biohazards and toxins used (CBS3 4.7.3)

  Yes

  No

  N/A

• 39. Work Surfaces Routinely Decontaminated (CBS3 4.7.2)

  Yes

  No

  N/A

• 39.1. Disinfectant is effective against agents being used (CBS3 4.4.18)

  Yes

  No

  N/A

• 40. Samples opened outside containment zones (CBS3 4.5.13)

  Yes

  No

  N/A

• 41. Regulated materials are to be inactivated with a validated and routinely verified method prior to removal from the containment zone for use at a lower containment level. (CBS3 4.5.18)

  Yes

  No

  N/A

• 42. Work may produce infectious aerosols or aerosolized toxins

  Yes

  No

  N/A

• 42.1. Only in BSC (CBS3 4.5.20)

  Yes

  No

  N/A

• 42.1.1. Other means of containment (CBS3 4.5.20)

  Yes

  No

  N/A

• 43. High concentrations or High volumes of infectious material

  Yes

  No

  N/A

• 43.1. Only in BSC (CBS3 4.5.20)

  Yes

  No

  N/A

• 43.1.1. Other means of containment (CBS3 4.5.20)

  Yes

  No

  N/A

• 44. Personnel know to report incidents to the supervisor (CBS3 4.8.5)

  Yes

  No

  N/A

• 45. Supervisor knows to report incidents to EHS (CBS3 4.8.9)

  Yes

  No

  N/A

Documentation

• 46. SOP: PPE requirements (CBS3 4.1.10)

  Yes

  No

  N/A

• 47. SOP: Entry/Exit (CBS3 4.1.10)

  Yes

  No

  N/A

• 48. SOP: Use of BSCs or other Containment Devices (CBS3 4.1.10)

  Yes

  No

  N/A

• 48.1. Prevents the inadvertent spread of contamination from items removed from the BSC after handling infectious material or toxins. (CBS3 4.5.1)

  Yes

  No

  N/A

• 48.2. Decontaminated before maintenance

  Yes

  No

  N/A

• 49. SOP: Animal Work Considerations (CBS3 4.1.10)

  Yes

  No

  N/A

• 50. SOP: Decontamination (CBS3 4.1.10)

  Yes

  No

  N/A

• 51. SOP: Waste Management (CBS3 4.1.10)

  Yes

  No

  N/A

• 51.1. Liquids decontaminated before entering sewer (CBS3 4.7.6)

  Yes

  No

  N/A

• 52. SOP: movement and transportation of infectious material and toxins (CBS3 4.1.10 and CBS3 4.5.19)

  Yes

  No

  N/A

• 53. SOP: Other tasks (CBS3 4.1.10)

  Yes

  No

  N/A

• 54. Training and refresher training records for all staff (CBS3 4.9.4)

  Yes

  No

  N/A

• 55. Is the Biohazard Inventory on Bio-permits up to date? (CBS3 4.9.5)

  Yes

  No

  N/A

• 56. Monthly self-inspection checklists (CBS3 4.9.8 and CBS3 5.1.2)

  Yes

  No

  N/A

• 57. Import permits & records (2 years past disposal) (CBS3 4.9.1)

  Yes

  No

  N/A

• Responsible Person (or designate)

  Sign

• Inspector

  Sign