Embrex Vaccination Audit

• Location
  Address

• Conducted on

  Date

Vaccine Room

• How is the cleanliness of the room?

  Good

  Fair

  Poor

  N/A

• Is the Daily Reconciliation paperwork complete and available?

  Compliant

  Non-Compliant

  N/A

• Are the proper S.O.P posted?

  Compliant

  Non-Compliant

  N/A

• Are the Nitrogen Levels measured and posted?

  Compliant

  Non-Compliant

  N/A

• Is the current vaccination mix sheet displayed?

  Compliant

  Non-Compliant

  N/A

• What vaccines/dose are being administered at the time of the audit?

• Is the dose calculation versus expected correct?

• Comments:

• Are hands washed prior to mixing?

  Compliant

  Non-Compliant

  N/A

• Is P.P.E. worn during the mixing of vaccine?

  Yes

  No

  N/A

• What PPE is worn?

What is the temperature of the thaw bath (Goal is 80F or 26.7C)?

• Is sanitizer used in the thaw bath?

  Yes

  No

  N/A

• What sanitizer is used?

• How much of the sanitizer is administered?

• Is the number of vials thawed at one time per bath 4 or less?

  Compliant

  Non-Compliant

  N/A

• How many vials were in the thaw bath at one time?

• How many seconds between placement and removal of vaccines from thaw bath?

• How many seconds between removal from thaw bath and complete placement in diluent?

• Is the vaccine injected into diluent bag slowly?

  Compliant

  Non-Compliant

  N/A

• Is the diluent bag agitated properly?

  Compliant

  Non-Compliant

  N/A

• Check 4 random nitrogen dewars for fullness

Dewar

• Level:

• Is the level adequate?

  Compliant

  Non-Compliant

  N/A

• Comments

Table/Crew #

• How many setter machines are leveled ahead of processing?

• Is this number in compliance with our goal?

  Compliant

  Non-Compliant

  N/A

• Is chlorine tank mixed and full?

  Compliant

  Non-Compliant

  N/A

• What is the level of chlorine when tested?

• Is the level in compliance with our goal?

  Compliant

  Non-Compliant

  N/A

• Are the vaccine lines air-free?

  Compliant

  Non-Compliant

  N/A

• is there leakage around the toolings?

  Compliant

  Non-Compliant

  N/A

• Are the heads dropping eggs?

  Compliant

  Non-Compliant

  N/A

• Are there cool packs in the vaccine hanger?

  Compliant

  Non-Compliant

  N/A

• What is the average transfer age?

• How often is the QC plate run?

• Is the table QC sheet being completed?

  Compliant

  Non-Compliant

  N/A

• How often is it completed?

• Are the completions initialized?

  Compliant

  Non-Compliant

  N/A

• Was a QC plate run for demonstration purposes during this audit?

  Yes

  No

  N/A

• Did all wells have the proper amount or sanitizer present?

  Compliant

  Non-Compliant

  N/A

• What was the number of wells with inadequate amounts?

• Was the problem corrected?

  Compliant

  Non-Compliant

  N/A

• Did all wells have the proper amount of vaccine present?

  Compliant

  Non-Compliant

  N/A

• What was the number of wells with inadequate amounts?

• Was the problem corrected?

  Compliant

  Non-Compliant

  N/A

• Were all the needles straight (not bent)?

  Compliant

  Non-Compliant

  N/A

• How many needles were bent?

• Instructions: Check 3 (MS) or 2 (SS) stacks per crew. Select stacks from different breeder flock sources.

Crew Stack

• Machine:

• Breeder #:

• Breeder Age:

• What is the percentage of transfer cracks for this stack?

• What is the overall percentage transfer cracks for this crew?

• Is the percentage at or below company goal?

  Compliant

  Non-Compliant

  N/A

• Comments:

Machine #

• Check 5 suction cups for cleanliness by removing the cups to look at connection point and feeling the inside for debris.

• Number of connection points clean?

• Number of inside clean?

• Remove 5 toolings and check for cleanliness.

• Number of toolings clean?

• Is there alcohol in the sanitizer carboy tank?

  Compliant

  Non-Compliant

  N/A

• Check one line to confirm alcohol prime is in the lines?

  Compliant

  Non-Compliant

  N/A

• Are carboy tanks stored upside down?

  Compliant

  Non-Compliant

  N/A