OGTR PC1 Laboratory Checklist - duplicate

• Audit Title

• Document No.

• Client / Site

• Conducted on

  Date

• Prepared by

• Location
  Address

• Personnel

• What biological work is undertaken within the facility?

• Are risk group 2 microorganisms used in the facility?

  Yes

  No

  N/A

• is Genetically Modified material in use or stored within the facility?

  Yes

  No

  N/A

The Facility

• Secure from entry of unauthorized personnel

  Yes

  No

  N/A

• Is there signage outside the facility indicating the containment level?

  Yes

  No

  N/A

• Are biohazard stickers attached to all entries to the facility?

  Yes

  No

  N/A

• Maintained so that it is a fully enclosed space, bound by walls, doors, windows, ceilings.

  Yes

  No

  N/A

• Surfaces are maintained so that they continue to be smooth, impermeable to water, cleanable and resistant to damage by cleaning agents and/or disinfectants in use:<br>- walls, floors and benches<br>- any other surface where decontamination is likely or decontamination is required.

  Yes

  No

  N/A

• Open spaces between and under benches, cabinets and equipment are accessible for decontamination.

  Yes

  No

  N/A

• Window furnishings are not located next to major sources of heat or potential fire hazards, such as hot plates or Bunsen burners

  Yes

  No

  N/A

• Bench tops can withstand the heat generated by general laboratory procedures, e.g flaming loops and heating of media

  Yes

  No

  N/A

• Is benchcoat used?

  Yes

  No

  N/A

• Is benchcoat replaced after spills?

  Yes

  No

  N/A

• Does the facility have a pest control system in place?

  Yes

  No

  N/A

• Furniture appropriate for the laboratory and the safety of laboratory users, and containment of microorganisms, e.g height adjustable stool with smooth, impervious seat covering to facilitate cleaning?

  Yes

  No

  N/A

• Are office areas outside the facility?

  Yes

  No

  N/A

Equipment

• Handwash basin, with hands free operation, or suitable alternate available.

  Yes

  No

  N/A

• Eye wash facilities available (plumbed or single use) maintained and flushed regularly.

  Yes

  No

  N/A

Biological Safety Cabinets

• Are BSC class I or II cabinets present for the containment of aerosols?

  Yes

  No

  N/A

• Have BSC been tested within the last 12 months?

  Yes

  No

  N/A

• Are the last test records available?

  Yes

  No

  N/A

Decontamination

• Are disinfectants effective against GMOs available in the facility for decontamination?

  Yes

  No

  N/A

• What decontaminants are being used?

• Are the decontaminants suitable for the work undertaken?

• Is there appropriate labeling such as volumes and expiration dates?

  Yes

  No

  N/A

• Are the decontaminants within the expiry date?

  Yes

  No

  N/A

• Are autoclaves used for the decontamination of waste prior to disposal?

  Yes

  No

  N/A

• - are autoclaves calibrated annually and are records for last 5 years available?

  Yes

  No

  N/A

• - what validation methods are used?

  Yes

  No

  N/A

• - are autoclaves validated monthly and are records for the last 12 months available?

  Yes

  No

  N/A

• Staff are trained to perform decontamination.

  Yes

  No

  N/A

• Benches are decontaminated at the end of work

  Yes

  No

  N/A

• Are items that are frequently touched (fridges, door handles, keyboards etc) decontaminated frequently?

  Yes

  No

  N/A

• Equipment decontaminated before removal from the facility.

  Yes

  No

  N/A

Waste

• How is waste disposed of?

• Is waste adequately segregated?

  Yes

  No

  N/A

• Are GMO waste bins available?

  Yes

  No

  N/A

• How is GMO waste disposed of?

• Are there GMO waste decontamination records available?

  Yes

  No

  N/A

Backflow prevention

• Does the facility have backflow protection?

  Yes

  No

  N/A

• Has the backflow prevention device been tested annually and are records available?

  Yes

  No

  N/A

Behaviour Requirements

• The preparation, storage or consumption of food and drink, smoking, shaving, chewing gum, inserting items into mouths, and the application of cosmetics or contact lenses is prohibited within the laboratory

  Yes

  No

  N/A

• Mouth pipetting is prohibited

  Yes

  No

  N/A

• Long hair (below shoulders) is tied back

  Yes

  No

  N/A

• Personal devices (e.g. earphones, mobile phones) are not handled with gloves and are not used in locations where they could become contaminated.

  Yes

  No

  N/A

• Where contamination is suspected, does decontamination occur prior to items leaving the laboratory?

  Yes

  No

  N/A

• Facility doors are closed while dealings are in progress.

  Yes

  No

  N/A

• Procedures are in place to ensure non-GMO work is not cross contaminated and procedures are documented.

  Yes

  No

  N/A

• Personal protective clothing is worn at all times inside the laboratory

  Yes

  No

  N/A

• Lab coats appropriately stored (ie. not on chairs/benches).

  Yes

  No

  N/A

• Windows are closed when work with GMOs is being conducted.

  Yes

  No

  N/A

• Production of aerosols is minimized, particularly when work is carried out on an open bench

  Yes

  No

  N/A

• Dissemination of microbiological material shall be minimized while flamming a wire loop, by drawing from cooler to hotter parts of the flame or by using disposable loops

  Yes

  No

  N/A

• Microbial cultures are clearly labeled, dated and transferred to a dedicated storage location when not in use

  Yes

  No

  N/A

• Diagnostic kits, control sera, and products manufactured from microbiological sources are handled using appropriate safety measures to avoid contact and spread of infectious microorganisms

  Yes

  No

  N/A

• Cultures of spore-producing microoragnisms are covered and sealed to prevent dispersal.

  Yes

  No

  N/A

Storage

• All cultures of GMO's are clearly labelled.

  Yes

  No

  N/A

• Are there records onsite for all GMOs

  Yes

  No

  N/A

• Storage of GMOs outside of facility are doubled contained.

  Yes

  No

  N/A

• Fridges/Freezers used for storage external to facility are labelled with biohazard symbol.

  Yes

  No

  N/A

• GMO signage with storage details on fridges/freezers

  Yes

  No

  N/A

Transport

• Is any biological material transported out of the facility?

  Yes

  No

  N/A

• If so, provide details

Centrifuges

• Is there any centrifugation of infectious material?

  Yes

  No

  N/A

• If so, are rotors or buckets sealed?

  Yes

  No

  N/A

Sharps

• Is the use of sharps minimized?

  Yes

  No

  N/A

• Are sharps containers available?

  Yes

  No

  N/A

• Are sharps handled safely?

  Yes

  No

  N/A

• Do they comply with AS4031?

  Yes

  No

  N/A

Emergency procedures

• Spill kits/appropriate spill containment material available.

  Yes

  No

  N/A

• Spill procedures documented and available.

  Yes

  No

  N/A

• Are biohazardous spill kits available and in date

  Yes

  No

  N/A

Records

• Staff laboratory training (PC1, Chemical Safety modules)

  Yes

  No

  N/A

• Onsite induction

  Yes

  No

  N/A

• Staff handling GMOs completed the Biosafety module

  Yes

  No

  N/A

• Risk management

  Yes

  No

  N/A

• Laboratory procedures

  Yes

  No

  N/A

• Emergency procedures

  Yes

  No

  N/A

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