Biosafety Level 2 Laboratory Inspection

• Biosafety Level 2 Laboratory Inspection

• Principal Investigator

• Building and room numbers

• Lab Representative

• Department

• Conducted on

  Date
• Location
  Address

• Prepared by

Facilities signage and practices

• Access to lab area restricted (I.e., controlled by lab supervisor)?

  Yes

  No

  N/A

• Biohazard signage present at lab entrance

  Yes

  No

  N/A

• Bench tops impervious to water and resistant to chemicals and heat?

  Yes

  No

  N/A

• Non-carpeted floors and lab furniture is made of non-porous materials

  Yes

  No

  N/A

• Evidence of food or drink in laboratory area?

  No

  Yes

  NA

• Emergency response procedures posted

  Yes

  No

  N/A

Housekeeping

• Lab is maintained in a clean/sanitary condition with no evidence of unattended spills and/ or other housekeeping concerns?

  Yes

  No

  N/A

• Evidence of pest management issues?

  No

  Yes

  NA

• Animals and plants not associated with work present in work areas?

  No

  Yes

  NA

• Lab equipment decontaminated routinely ( no evidence of unattended splashes and spills)

  Yes

  No

  N/A

Shared facilities or equipment housekeeping

• Are there any shared freezers or refrigerators?

Freezers

• Equipment clean and free of housekeeping concerns?

  Yes

  No

  N/A

• Samples are properly labeled and stored in leak-proof containers?

  Yes

  No

  N/A

• Responsible group or PI?

• Are there any shared cold rooms?

Cold rooms

• Equipment clean and free of housekeeping concerns?

  Yes

  No

  N/A

• Samples are properly labeled and stored in leak-proof containers?

  Yes

  No

  N/A

• Responsible group or PI?

• Are there any shared incubators?

Incubators

• Equipment clean and free of housekeeping concerns?

  Yes

  No

  N/A

• Samples are properly labeled and stored in leak-proof containers?

  Yes

  No

  N/A

• Responsible group or PI?

Sharps and biological waste disposal practices

• Needles and other sharps are disposed of in appropriate sharps container?

  Yes

  No

  N/A

• Needles did not appear to be intentionally bent, broken, or recapped (unless exempted)?

  No

  Yes

  NA

• Biological waste containers were properly labeled, leak proof, lined with a biohazard bag, and lids were available if applicable?

  Yes

  No

  N/A

Hygiene and Primary containment measures

• Hand washing sink including soap and hand towels available

  Yes

  No

  N/A

• Eyewash stations

Station

• Free of obstructions and easily accessible?

  Yes

  No

  N/A

• Functional?

  Yes

  No

  N/A

• Documentation showing station has been flushed/tested monthly

  Yes

  No

  N/A

• Date of last test/flush

  Date

• BSCs

Cabinet

• Type of cabinet

  Select Clean Bench Class I Class IIa Class IIb1 Class IIb2 Class III

  • Clean Bench
  • Class I
  • Class IIa
  • Class IIb1
  • Class IIb2
  • Class III

• Unless otherwise noted as "not intended for use with BSL-2 materials", BSC has been certified within the last year?

  Yes

  No

  N/A

• Date of last Certification

  Date

• BSC is located away from doors, high traffic areas, and other airflow disruptions have been minimized?

  Yes

  No

  N/A

• Vacuum lines are properly filtered / liquid waste decontamination traps are properly housed in spill proof container?<br>

  Yes

  No

  N/A

PPE

• Gloves readily available?

  Yes

  No

  N/A

• Lab coats readily available?

  Yes

  No

  N/A

• Eye protection readily available?

  Yes

  No

  N/A

Biosafety Resources and Documentation

• Have any new personnel been added who require BSL-2 or BBP training?

  No

  Yes

  NA

• Name and UT NetID

• Biosafety manual is readily available

  Yes

  No

  N/A

• Location?

• Biological spills procedures accessible

  Yes

  No

  N/A

• Site specific training documented

  Yes

  No

  N/A

• Does laboratory use a shipping designee?

  No

  Yes

  NA

• Responsible personnel?

• Are laboratory personnel working with human derived tissues, bodily fluids or cell lines that would require BBP training?

  No

  Yes

  NA

Blood Borne Pathogens

• Exposure control plan is accessible?

  Yes

  No

  N/A

• Are there any new personnel that require BBP training?

  No

  Yes

  NA

• Name and UT NetId?

• Are there new personnel that have not been informed of their right to vaccination for Hepatitis B ?

  No

  Yes

  NA

• Name and UT NetID?

Questionnaire

• Was a lab representative available?

  Yes

  No

  N/A

BSL-2 practices

• All procedures that may generate aerosols are conducted in BSC

  Yes

  No

  N/A

• Procedures are designed to minimize the generation of aerosols and/or splashes?

  Yes

  No

  N/A

• Personnel were familiar with the proper decontamination strategies for biological waste (e.g., cultures, stocks) prior to disposal?

  Yes

  No

  N/A

• Workers practice good hygiene/ hand washing procedures?

  Yes

  No

  N/A

• Personnel are aware of how to properly store and transport samples (I.e., capped leak-proof primary container, with leakproof secondary)?

  Yes

  No

  N/A

Emergency procedures

• Personnel are familiar with post exposure evaluation and follow-up

  Yes

  No

  N/A

• Personnel familiar with spills procedure?

  Yes

  No

  N/A

Summary information

• Comments

• Mandatory Corrections

• Deficiencies must be corrected by

• Signature of laboratory representative

  Sign

• This Biosafety Level 2 (BSL-2) Inspection is in accordance with requirements outlined in the CDC/NIH Biosafety in Microbiological and Biomedical Laboratories 5th ed document (BMBL) and/or other applicable regulatory standards per Institutional Biosafety Committee and Biosafety Office policy.
  Additional Information on the BMBL, BSL-2 requirements, applicable regulatory standards, and lab inspection parameters can be found at http://www.biosafety.utk.edu. Please contact the Office of Biosafety if you have any questions or concerns regarding this evaluation. Thank you in advance for your cooperation in ensuring biological safety and research compliance.

• Legend:
  
  Pass: Score >85% No, or minor deficiencies were noted that were resolved at the time of inspection. Remedial actions are not required at this time.
  
  Provisional Pass: Score >85% Deficiencies were noted; one or more requiring prompt remedial action (Noted under Mandatory Corrections). A mandatory correction is issued when: 1) there is a violation of a U.S. Federal, state, or local regulatory standard; and/or 2) the biosafety inspector has deemed it a risk to personnel safety or biological containment. Remedial action must be taken (or arranged) by the date indicated. Failure to comply will result in a 'FAIL'. Contact the Biosafety Office if you would like further explanation or guidance in resolving the noted issues.
  
  FAIL: Score <85% Numerous or significant deficiencies were noted. Remedial actions must be taken (or arranged by) the date indicated. If corrections have not been taken by that date, the department head and IBC will be consulted to determine the appropriate disciplinary action. Contact the Biosafety Office if you would like further explanation or guidance in resolving the noted issues.