Biosafety Level 2 Laboratory Inspection

• Principal Investigator(s)

• IBC #

• Protocol approved

  Yes

  No

  N/A

• Approval date

  Date

• Review date

  Date

• Department/Division

• Building and room numbers

• Lab Representative

• Phone number

• Infectious agent(s)

• Biosafety level

• Conducted on

  Date

• Prepared by

• Biosafety officer(s)

  Select John Bivona, R.B.P. R. Allen Helm, Ph.D., R.B.P. Lauriane Quenee, Ph.D. Hsiang-Ming (Anthony) Wang, Ph.D., MBA, SM(NRCM)

  • John Bivona, R.B.P.
  • R. Allen Helm, Ph.D., R.B.P.
  • Lauriane Quenee, Ph.D.
  • Hsiang-Ming (Anthony) Wang, Ph.D., MBA, SM(NRCM)

• Biosafety officer signature

  Sign

A. Standard Microbiology Practices

• Access to lab area restricted (I.e., controlled by lab supervisor)?

  Yes

  No

  N/A

• Good hand washing practice including soap availability

  Yes

  No

  N/A

• Evidence of food or drink in laboratory area

  No

  Yes

  NA

• Sign of mouth pipetting

  No

  Yes

  NA

• Device/practice to minimize aerosol generation

  Yes

  No

  N/A

• Lab is maintained in a clean/sanitary condition. Work surface is decontaminated daily with no evidence of unattended spills and/ or other housekeeping concerns?

  Yes

  No

  N/A

• Containers are properly labelled

  Yes

  No

  N/A

B. Special Practices

• Door have locks and is closed when BSL2 work is in progress

  Yes

  No

  N/A

• Health surveillance program in place

  Yes

  No

  N/A

• Select Immunization Baseline serum High risk personnel

  • Immunization
  • Baseline serum
  • High risk personnel

• Comments

• Biohazard signage present at lab entrance

  Yes

  No

  N/A

• Biohazard stickers are attached to equipment

  Yes

  No

  N/A

• BSL-2 policies and procedures established

  Yes

  No

  N/A

• Lab members know how to access the policies including IBC protocols and Biosafety manual

  Yes

  No

  N/A

• Microbiological safety audit completed/updated

  Yes

  No

  N/A

• Use of containers that prevent leakage of material

  Yes

  No

  N/A

• Emergency response procedures/ spill protocol posted

  Yes

  No

  N/A

• Approved disinfectant are used, labelled, and dated

  Yes

  No

  N/A

C. General housekeeping

• Evidence of pest management issues?

  No

  Yes

  NA

• Insect/rodent control in place?

  Yes

  No

  N/A

• Animals and plants not associated with work present in work areas?

  No

  Yes

  NA

• Lab equipment decontaminated routinely ( no evidence of unattended splashes and spills)

  Yes

  No

  N/A

D. Sharps and biological waste disposal practices

• Sharps used in the lab

  Yes

  No

  N/A

• Types of sharps used

  Select Needles Devices Glassware

  • Needles
  • Devices
  • Glassware

• Sign of recapping needle

  No

  Yes

  NA

• Needles and other sharps are disposed of in appropriate sharps container?

  Yes

  No

  N/A

• Waste decontamination prior to disposal

  Yes

  No

  N/A

• Autoclaving

  Yes

  No

  N/A

• On-site disposal (boxes from EH&S)

  Yes

  No

  N/A

• Biological waste containers were properly packed and labelled?

  Yes

  No

  N/A

E. PPE

• Gloves readily available?

  Yes

  No

  N/A

• Types of gloves available

  Select Latex Nitrile Other

  • Latex
  • Nitrile
  • Other

• Comments

• Lab coats readily available?

  Yes

  No

  N/A

• Eye protection readily available?

  Yes

  No

  N/A

F. Facilities & primary containment

• Sink in each lab

  Yes

  No

  N/A

• Bench tops impervious to water and resistant to chemicals and heat?

  Yes

  No

  N/A

• Non-carpeted floors and lab furniture is made of non-porous materials

  Yes

  No

  N/A

• Windows fitted with fly screens

  Yes

  No

  N/A

• Eyewash stations

Station

• Location

• Free of obstructions and easily accessible?

  Yes

  No

  N/A

• Functional?

  Yes

  No

  N/A

• Documentation showing station has been flushed/tested weekly

  Yes

  No

  N/A

• Date of last test/flush

  Date

• BSCs

Cabinet

• Type of cabinet

  Select Clean Bench Class I Class IIa Class IIb1 Class IIb2 Class III

  • Clean Bench
  • Class I
  • Class IIa
  • Class IIb1
  • Class IIb2
  • Class III

• Unless otherwise noted as "not intended for use with BSL-2 materials", BSC has been certified within the last year?

  Yes

  No

  N/A

• Date of last Certification

  Date

• BSC is located away from doors, high traffic areas, and other airflow disruptions have been minimized?

  Yes

  No

  N/A

• Vacuum lines are properly filtered / liquid waste decontamination traps are properly housed in spill proof container?<br>

  Yes

  No

  N/A

• Location

• Make & model#

• S/N

Centrifuge

• Used with biohazard?

  Yes

  No

  N/A

• Aerosol proof lids available

  Yes

  No

  N/A

G. Biosafety Resources and Documentation

• Have any new personnel been added who require BSL-2 or BBP training?

  No

  Yes

  NA

• Name and UC cNetID

• Biosafety manual is readily available

  Yes

  No

  N/A

• Location?

• Does laboratory use a shipping designee?

  No

  Yes

  NA

• Responsible personnel?

• Are laboratory personnel working with human derived tissues, bodily fluids or cell lines that would require BBP training?

  No

  Yes

  NA

Blood Borne Pathogens

• Exposure control plan is accessible?

  Yes

  No

  N/A

• Are there any new personnel that require BBP training?

  No

  Yes

  NA

• Name and UC cNetID

• Are there new personnel that have not been informed of their right to vaccination for Hepatitis B ?

  No

  Yes

  NA

• Name and UC cNetID

H. Questionnaire

• Was a lab representative available?

  Yes

  No

  N/A
Interviews

BSL-2 practices

• All procedures that may generate aerosols are conducted in BSC

  Yes

  No

  N/A

• Procedures are designed to minimize the generation of aerosols and/or splashes?

  Yes

  No

  N/A

• Personnel were familiar with the proper decontamination strategies for biological waste (e.g., cultures, stocks) prior to disposal?

  Yes

  No

  N/A

• Workers practice good hygiene/ hand washing procedures?

  Yes

  No

  N/A

• Personnel are aware of how to properly store and transport samples (I.e., capped leak-proof primary container, with leakproof secondary)?

  Yes

  No

  N/A

Emergency procedures

• Personnel are familiar with post exposure evaluation and follow-up

  Yes

  No

  N/A

• Personnel familiar with spills procedure?

  Yes

  No

  N/A

Toxins

• Select the toxins used

  Select Abrin** Aerolysin Botulinum neurotoxin** b-bungarotoxin Clostridium difficile enterotoxin Clostridium perfringens toxin Conotoxin** Diacetoxyscirpenol** Diphtheria toxin Listeriolysin Modeccin Pertussis toxin Pneumolysin Pseudomonas aeruginosa toxin A Ricin** Saxitoxin** Shiga toxin Shigella dysenteriae neurotoxin Staphylococcus enterotoxins** Steeptolysin O/S T-2 toxin** Taipoxin Tetanus toxin Teytodotoxin** Volkensin Yersinia pest is murine toxin

  • Abrin**
  • Aerolysin
  • Botulinum neurotoxin**
  • b-bungarotoxin
  • Clostridium difficile enterotoxin
  • Clostridium perfringens toxin
  • Conotoxin**
  • Diacetoxyscirpenol**
  • Diphtheria toxin
  • Listeriolysin
  • Modeccin
  • Pertussis toxin
  • Pneumolysin
  • Pseudomonas aeruginosa toxin A
  • Ricin**
  • Saxitoxin**
  • Shiga toxin
  • Shigella dysenteriae neurotoxin
  • Staphylococcus enterotoxins**
  • Steeptolysin O/S
  • T-2 toxin**
  • Taipoxin
  • Tetanus toxin
  • Teytodotoxin**
  • Volkensin
  • Yersinia pest is murine toxin

• Quantity

  Yes

  No

  N/A

• IBC protocol available

  Yes

  No

  N/A

• IBC #

Summary information

• Comments

• Mandatory Corrections

• Deficiencies must be corrected by

• Signature of laboratory representative

  Sign

• This Biosafety Level 2 (BSL-2) Inspection is in accordance with requirements outlined in the CDC/NIH Biosafety in Microbiological and Biomedical Laboratories 5th ed document (BMBL), NIH guidelines, and/or other applicable regulatory standards per Institutional Biosafety Committee and Office of Biosafety policy.
  Additional Information on the BMBL, BSL-2 requirements, applicable regulatory standards, and institutional Biosafety Manual can be found on https://biologicalsafety.uchicago.edu/. Please contact the Office of Biological Safety if you have any questions or concerns regarding this evaluation. Thank you in advance for your cooperation in ensuring biological safety and research compliance.