Title Page
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Document No.
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Inspection reason
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Inspector
- DSO
- Lab user - PI
- Lab user - Researcher
- Technical inspection
- Head of Department
- Faculty inspector
- College Inspector
- External Inspector
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Laboratory / area (e.g. B630, RSM 4.21)
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Lead user / Contact
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Conducted on
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Prepared by
Lab safety Inspection - Outstanding actions
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Has the previous inspection in the area been reviewed
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Are there any outstanding actions
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Please detail outstanding action points. These actions must be completed, with any raised during this inspection, before sign off is possible.
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The previous inspection and all actions must be reviewed before sign off of this inspection is possible.
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Are there any outstanding SALUS reports or actions for the area?
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Is there a suitable plan in place to close out the actions?
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Lab safety Inspection - Facilities & housekeeping
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L.A Facilities & Housekeeping
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L.A.1 Access to laboratory is secure and restricted to appropriate personnel
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L.A.2 Appropriate PPE is available and in use ( PPE includes safety glasses, laboratory coat and suitable gloves)
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L.A.3 A variety of appropriate protective gloves are available
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L.A.4 Standard operating procedures are displayed next to equipment
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L.A.5 Warning signs for laser, radiation, biological or other hazards are prominently posted
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L.A.6 Food and drink are not stored or consumed in laboratories
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L.A.7 There is a landline telephone available within the lab
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L.A.8 Is the laboratory Hazard assessment present and up to date?
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Please ensure this is completed and/or updates as soon as possible
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Visit lab hazard assessment database
https://imperiallondon.sharepoint.com/sites/foe/Bioengineering/LIMS/Lists/Laboratory%20hazard%20assessment/AllItems.aspx -
Is the lab induction form up to date and logged in the database?
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Visit induction database
https://imperiallondon.sharepoint.com/sites/foe/Bioengineering/LIMS/Laboratory%20induction%20forms/Forms/AllItems.aspx -
is the Safety folder up to date?
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L.B. Housekeeping
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L.B.1 Aisles/exits are clear
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L.B.2 Spill kits, supplies and decontamination material are available for the hazards present
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L.B.3 Spill procedures are clearly displayed
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Lab bench housekeeping
Element
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Bench number/ designation
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L.B.4 Benches only occupied by chemicals/equipment in use
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L.B.5 Storage of items on floors is kept to a minimum
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L.B.6 Storage of heavy items above shoulder height is avoided
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L.B.7 (Official) Signs are attached to unattended experiments with appropriate details
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Media/picture
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L.C General Waste Management (black and orange bags, general laboratory liquids and items)
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L.C.1 All waste is disposed of appropriately
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L.C.2 Hazardous wastes and materials are contained and do not drain to sewer
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L.C.3 Hazardous waste is clearly labelled with waste description, biohazard symbols if required and date produced. The waste label includes the waste generator contact details (name, department/school/unit and contact number) and if the waste has had any on-site treatment or testing. A storage stable risk assessment has been completed
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L.C.4 Wastes are segregated according to hazard, treatment requirements and to optimise recycling opportunities
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L.C.5 Wastes are stored in fit for purpose containers (e.g. wheelie bin) that are able to withstand storage conditions and transport
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L.C.6 Wastes are stored at appropriate temperatures
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L.C.7 There are sufficient and appropriate waste disposal containers
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L.C.8 Wastes are disposed of regularly and volumes are kept to a minimum
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L.D. Electrical equipment
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L.D.1 Laboratory equipment, including cords and leads, are in good working order
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L.D.2 There are sufficient general-purpose outlets (GPOs) for the equipment
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L.D.3 Double adaptors; piggy-back plugs are not in use
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L.D.4 Extension leads are only used for low power connections
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L.D.5 Extension leads with overload protection are used if sufficient GPOs are not available
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L.D.6 Equipment is appropriately CE marked
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L.D.7 Fittings are protected against external damage
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L.D.8 Equipment that may be left on unattended has a manual cut-off switch
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L.D.9 Electrical equipment is tested at appropriate intervals (PAT tested)
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L.D.10 Any modified/designed (in-house) electrical equipment has been risk assessed and certified as safe by the departmental electrical safety officer (e.g. has an approved notice)
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L.D.11 Out of Service tags for faulty electrical equipment are available and used
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L.D. 12 Uninterruptable Power Supply (if present) has been inspected, tested and monitored. Have the batteries (battery pack) been inspected (or replaced) in the last 2 years? Any UPS in storage must have the battery pack disconnected. (Hazard Alert 2012)
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L.E. Refrigerators/freezers/cool rooms
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L.E.1 Refrigerators, freezers, cool rooms are appropriately labelled (e.g. spark free/intrinsically safe)
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L.E.2 Fridges, freezers etc. are regularly defrosted (particularly -80c)
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L.E.3 Flammable liquids are only stored in fridges/freezers that are intrinsically safe (spark free)
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L.F. Environmental issues
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L.F.1 Is the temperature appropriate within the area
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L.F.2 Is the lighting appropriate and all functioning correctly
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L.F.1 Chemical waste bottles are clearly labelled and assessed for storage stability
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L.F.2 Chemical containers are cleaned prior to recycling or disposal:<br>- Triple rinsed and labels and lids removed before placing in the bottle and can recycling bins<br>- Containers with oily or persistent residues are washed with detergent and labels removed before placing in the bottle and can recycling bins
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L.F.3 Contaminated glassware that is not cleaned as above is treated as a prescribed industrial waste
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L.F.4 There is no evidence of incorrect disposal down sinks, ie discoloration, staining, odorous sinks, etc
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L.F.5 Hoses in sinks are short to prevent backflow contamination of the water supply
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L.F.6 There are no odours, dust or smoke in the area
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L.F.7 All service areas, where chemicals are used and dispensed or where wastes are stored are suitably bunded/protected from leakage into storm water or sewer drains.
Lab Safety Inspection - Specialist equipment
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L.F. Fume cupboards
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L.F.1 Fume cupboards are appropriate for the type of hazard (ie radiation, biological, etc)
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L.F.2 The fume cupboard is used with the sash at or below the recommended height
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L.F.3 The leading edge of the fume cupboard is clear of equipment and other items (also see the Guidelines on the use of Fume cupboards )
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L.F.4 The floor space in front of the hood, the main work area, is clear
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L.F.5 The fume cupboard is free of clutter and large equipment
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L.F.6 The doors and windows within 1 m of the cupboard are closed when the cupboard is used
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L.F.7 Emergency switches are clearly identified for power and gas supply
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L.F.8 The fume cupboard has been inspected and certified within the last 6-12 months
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Media
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L.G. Biosafety Cabinets
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L.G.1 The biosafety cabinet is clean and free of clutter (i.e. routine cleaning performed recently)
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L.G.2 The biosafety cabinet has been inspected and certified within the last 12 months
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Media
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L.H. Centrifuges
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L.H.1 The centrifuge and rotor is regularly inspected for safety
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L.H.2 The centrifuge and rota show no sign of spillage or wear
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L.I. Autoclaves
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L.I.1 The autoclave is regularly inspected and calibrated
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L.I.2 Autoclave records are retained and show that sterilisation was achieved
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L.J. Safety Equipment
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L.J.1 Eye wash facilities are tested routinely
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L.J.2 The eyewash station provides aerated water at an appropriate pressure
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L.J.3 The eyewash station is not blocked and is readily accessible
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L.K. Chemicals (general)
Chemical management
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L.K.1 Chemicals are registered on the departmental COSHH database
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COSHH database
https://share.imperial.ac.uk/foe/Bioengineering/LabInformation/TempCoshh/Forms/AllItems.aspx -
L.K.2 MSDS are available for chemicals
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L.K.3 Documented COSHH/risk assessments are available for chemical use
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L.K.4 Carriers are provided for large containers (>2L)
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L.K.5 Unattended reactions are clearly signed
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L.K.6 Hydrofluoric acid use is careful managed in accordance with safety Guidelines
Labelling of chemicals
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L.K.7 All chemicals are labelled clearly and unambiguously in accordance with the COSHH regulations 2002
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L.K.8 All reused containers have label removed or completely covered
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L.K.9 Decanted chemicals are appropriately labelled
Chemical storage
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L.K.10 All poisons and drugs are stored in a locked cabinet/safe
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L.K.11 Carcinogens are securely stored
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L.K.12 Chemicals not in use are placed in the correct storage location
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L.K.13 Chemical stocks are kept to a minimum. A standard laboratory is generally allowed 6-8 Winchesters each of solvents and acids
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L.K.14 Incompatible classes of chemicals are segregated (generally by dangerous goods class)
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L.K.15 Chemical containers are of the correct type and in good condition
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L.K.16 No food containers are in use for the storage of chemicals
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L.K.17 Unsealed containers of flammable waste are stored in fume cupboards or ventilated cupboards
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L.K.18 Incompatible chemicals stored in the same cupboard have separate spill containment (e.g. acids and bases)
Storage on open shelving
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L.K.19 All containers are <5 kg/L in size
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L.K.20 On shelves >1.5 m high all glass containers are <1kg/L
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L.K.21 The shelving is chemically resistant to the chemicals stored
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L.K.22 Plastic chemical containers are in good condition and not older than 5 years (or recommended by the manufacturer as a suitable life of the plastic. see date stamp on container)
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L.K.23 Bottles with liquid dispensers fitted are restrained from falling and accidental discharge
Storage in fire-proof cabinets
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L.K.24 All containers in cabinets are <25L in size
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L.K.25 The self-closing mechanisms of the doors are operating correctly
Storage in ventilated cupboards
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L.K.26 All containers in cupboards are <2kg/L in size
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L.K.27 Incompatible chemicals stored in the same cupboard have separate spill containment
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L.K.28 Ventilation is sufficient to prevent build-up of offensive odours
Evidence of chemical spills or incorrect disposal
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L.L. Compressed and fuel gases
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L.L.1 All the cylinders inside rooms are connected for use (ie no laboratory storage) flammable or toxic gas cylinders may only be used in the fume cupboard
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L.L.2 Gas cylinders are each securely chained with individual chains secured around the body of the cylinder and the chain ends attached to 2 separate securing points
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L.L.3 Cylinders, pipes & valves are protected from mechanical damage
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L.L.4 Strength of hoses & clamps attached to gas cylinder regulators are sufficient for maximum regulator delivery pressure
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L.L.5 Obsolete gas regulators are not in use
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L.L.6 The gas cylinder and major joints are regularly tested for leaks
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L.L.7 Flashback arresters are used in fuel gas supply lines
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L.L.8 Non-return (check) valves are fitted to all processes that exceed cylinder pressure
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L.L.9 Unusual gas systems have been inspected and approved by BOC
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L.L.10 There is a cylinder trolley available for carrying cylinders
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L.M. Cryogenic liquids
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L.M.1 Cryogenic liquids are located in well-ventilated areas
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L.M.2 Containers used for cryogenic liquids are designed specifically for that purpose
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L.M.3 Appropriate protective gloves and face shield are available
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L.M.4 Low oxygen alarm is present, calibrated and working
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L.N. Biological hazards
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L.N.1 Documented risk assessments (Bio1) for use of biological hazards are available
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L.N.2 Hand washing facilities are available.
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L.N.3 Biological materials are clearly labelled, dated and appropriately stored
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L.N.4 Appropriate disinfectants are available
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L.N.5 Appropriate waste handling procedures are in place (autoclaving, disinfecting etc.)
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L.N.6 Biohazardous waste and sharps bins are provided, where necessary
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L.N.7 The sharps containers are of single shell construction and safety compliant
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L.N.8 Separate facilities are provided for animal storage, cleaning, examination, etc.
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L.N.9 No items are stored on the floors
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L.N.10 Immunisations have been considered by staff and students (Hep B)
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L.N.11 Health Surveillance has been discussed with staff and students
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L.N.12 Glass pipettes have been considered for replacement with alternatives (e.g. plastic)
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L.N.13 Strong sealable containers are available to transport materials around the building or between buildings
Lab safety Inspection - Radiation, lasers
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L.O. Ionising radiation hazards
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L.O.1 A radiation safety officer has been appointed to review the radiation work and their details are displayed
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L.O.2 An appropriate radiation warning sign is displayed at the entrance
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L.O.3 Documented risk assessments and safe work procedures for use of ionising radiation are available
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L.O.4 Emergency procedures are available and reviewed annually
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L.O.5 Personal occupationally exposed to ionizing radiation are issued with a personal dosimetry device (e.g. TLD Badge)
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L.0.6 Radiation contaminated monitoring or x-ray leakage tests are regularly conducted and recorded
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L.P. Radioactive unsealed sources
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L.P.1 Documented risk assessments and safe work procedures for use of ionising radiation are available
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L.P.2 Work surfaces are impervious (wooden benches and tiled surfaces are unsuitable)
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L.P.3 Hand washing facilities are available
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L.P.4 Appropriate shielding material is available
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L.P.5 Secondary containment is available and utilised (i.e. spill trays)
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L.P.6 All sources of ionising radiation are labelled and stored in secure locations
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L. P.7 All storage locations of ionising radiation are labelled
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L.P.8 Appropriate monitoring equipment is available and has been regularly inspected/tested/calibrated.
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L.P.9 Decontamination/emergency procedures are clearly displayed
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L.P.10 Contamination checks are conducted before and after each procedure
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L.Q. Radioactive sealed sources
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L.Q.1 Documented risk assessments and safe work procedures for use of ionising radiation are available
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L.Q.2 Hand washing facilities are available
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L.Q.3 Appropriate shielding material is available
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L.Q.4 All sources of ionising radiation are labelled and stored in secure locations
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L.Q.5 All storage locations of ionising radiation are labelled
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L.Q.6 Appropriate monitoring equipment is available
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L.Q.7 Sealed radiation sources are maintained in good order
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L.T Lasers
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Laser Classification
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L.T.1 Laser laboratories housing class 3 and/or class 4 lasers are restricted and access is controlled
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L.T.2 A laser warning sign is clearly displayed at the entrance. This is a black symbol on a yellow background. The laser class and wavelength are also indicated.
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L.T.3 A laser safety officer is responsible for the area and their name and contact details are displayed
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L.T.4 Beam is restricted to the optics bench or equipment by a physical barrier
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L.T.5 Beam stops are installed
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L.T.6 Appropriate fail-safe devices to prevent accidental exposure are in place
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L.T.7 Documented risk assessments and safe work procedures for use of lasers are available
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L.T.8 Procedures (incl. emergency) are reviewed annually
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L.T.9 Wavelength-specific eye protection is available for laser operators
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L.S. Ultraviolet radiation
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L.S.1 Appropriate shielding and/or interlocks are in place (e.g. interlocks on germicidal lamps, and protective shields on transilluminators )
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L.S.2 Additional PPE is available (i.e. additional skin and eye protection may be required)
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X-Ray apparatus or sealed source apparatus
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L.R.1 Documented risk assessments and safe operating procedures for use of ionising radiation are available
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L.R.2 Appropriate shielding material is available
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L.R.3 Appropriate monitoring equipment is available
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L.R.4 Appropriate fail-safe devices to prevent accidental exposure are in place
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L.R.5 Appropriate labelling is in place
Designated laboratory inspection (annual)
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Designated laboratory
Documentation
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Are the following available for reference:
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Designated Laboratory Application Form Part A (Form DLA)
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Designated laboratory Application Form Part B (Health & Safety) (Form DLB)
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Are relevant COSHH & other relevant risk assessments available within the laboratory and logged in RADAR
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Radar Database
http://www.imperial.ac.uk/ict/apps/radar -
The relevant Standard Operating Procedure for transfer of animals
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SOPs covering specific techniques used in the work
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SOP listing
https://share.imperial.ac.uk/foe/Bioengineering/LabInformation/Lists/SOP%20list/AllItems.aspx
Document validity
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Have the Designated laboratory application Forms (Forms DL Part A and B) been signed off?
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Is the Project Licence Holder specified in the Project Registration form still in charge of the work?
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Have risk assessments been reviewed?
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Is the personnel list (PRF Form B, Section 12) complete & up-to-date
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Does the laboratory room number accord with the local CBS record?
Laboratory set-up
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Is the laboratory reserved exclusively for animal work?
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Hand washing facility in laboratory or adjacent
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Is there designated storage for ‘normal’ clothing outside of the laboratory?
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Are work surfaces impervious and in good condition?
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Is the work area screened from external view
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Is access security in place?
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Is the door closed during work
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Is there adequate segregation of animal from non-animal work?
LEV
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Are appropriate ventilated cabinets available and being used?
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Has there been a satisfactory documented engineer’s inspection in the past 6 months
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Is there an up-to-date record of monthly user airflow checks?
Personal protective equipment
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Are there adequate stocks of specific PPE available
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Are dedicated, colour-coded laboratory coats available
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Is PPE stored appropriately?
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Is PPE used correctly?
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Is there a maintenance record for any re-useable RPE
Transfer of animals
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Do the containers used accord with that specified in PRF Form B & the SOP?
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If bedding material is used, does it accord with that specified in PRF form B?
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Does the transport route used accord with that specified in PRF Form B
Holding arrangements
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Is holding time in the lab minimised?
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Is allergen containment during holding assured?
Conduct of work
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Is all work with animals whilst awake carried out in ventilated cabinet/ downdraft bench
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Is there a justification available for any open-bench work
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Is shaving conducted within an LEV cabinet
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If surgery is carried out on the open bench is draping or wetting of fur used?
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Is the culling method used compliant with the CoP?
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Are cadavers & waste materials promptly bagged
Hygiene & housekeeping
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Are hand washing arrangements adequate?
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Are laundering arrangements for animal lab coats in place and being used
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Is there an enclosed waste bin/ bag for used PPE/ biological waste
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Are work surfaces uncluttered and clean
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Is there enclosed storage available & used ?
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Are cleaning & waste disposal arrangements followed?
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Are appropriate cleaning materials & equipment available
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Are empty transport boxes/ IVCs returned to CBS
Personnel
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Are all potentially exposed enrolled for health Surveillance
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Are all aware of controls and following them
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Do all users know how to check cabinet airflow?
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Have competences been assessed
Overnight holding
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Are animals housed appropriately?
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Is the room used exclusively for animal work?
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Does the NACWO visit regularly
Lab safety Inspection - sign off
Sign Off
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On-site representative
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Auditor'signature
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Auditor 2 signature
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Auditor 3 signature
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Auditor 4 signature
