Quarterly Laboratory Audit Checklist

1.1 Work area not cluttered and no evidence of spills

1.2 Aisles not blocked (3' clearance)

1.3 No storage of equipment, chemicals, bottles or glassware on floor

1.4 Heavy items, glassware, and chemicals not stored above 1st shelf

1.5 No evidence of smoking, eating or drinking in lab

1.6 No excess combustible materials (empty boxes, etc.)

1.7 Machines and equipment do not have exposed sharp edges

1.8 Laboratory doors are kept close

1.9 Knee holes where researchers sit are unobstructed

1.10 Monthly lab inspection has been completed and available for viewing

2.1 Employees are wearing lab coats and safety glasses upon entry into lab areas

2.2 Appropriate footwear and no leg exposure in the lab

2.3 Appropriate gloves used when working (i.e. cryogenic or nitrile)

2.4 Safety glasses and lab coats available to visitors

2.5 Additional eye/face PPE (goggles or goggles w/face shield) worn when working with or working within 15' of corrosive materials (i.e. Sodium hydroxide >0.2N)

2.6 Disposable lab coats are available and disposed of properly after use

3.1 Eyewash / Safety Shower inspections are current

3.2 Eyewash / Safety Showers are not blocked. Eyewashes in lab sinks are unobstructed

3.3 Fire equipment is not blocked

3.4 Fire extinguisher inspections are current

3.5 Sprinklers not blocked (at least 18" of clearance underneath)

3.6 No storage within 18" of the ceiling in any laboratory (includes cold rooms and warm rooms)

3.7 Spill kit is available and stocked

3.8 Phones work, are unobstructed, and have emergency numbers posted

3.9 Emergency exits / alarm strobes are labeled and unobstructed

3.10 Personnel within the labs are aware of the actions they are supposed to take during a fire or other emergency

4.1 Chemicals segregated properly and are stored in proper cabinets

4.2 Secondary containers are used properly

4.3 Chemicals are closed and in good condition (no evidence of spills)

4.4 Peroxide formers labeled and tested within 3 months

4.5 Flammables are stored in approved cabinet or explosion proof refrigerators

4.6 All chemicals are properly labeled with GHS labels

4.7 Empty bottles are defaced and disposed of properly

4.8 Secondary containment used near sink

4.9 Storage cabinets are free of rust and corrosion

4.11 CMRs are stored in dedicated area and labeled

4.12 Drums containing hazardous liquids contain secondary containment

5.1 Human source material is tested and documentation of results is maintained in binder

5.2 Human / Non-Human Primate source log is maintained, updated, and accessible in laboratory

5.3 Hazard warning labels on containers of biohazardous materials and waste

5.4 Bench tops, sinks and other work areas are clean/decontaminated

5.5 All work with untested Human / Non-Human Primate source material takes place in BSL2+ designated areas

5.6 Hazardous drugs, cytotoxic agents, and novel compounds are stored in designated are labeled areas in the lab

5.7 Hazardous drugs, cytotoxic agents, and novel compounds are disposed of as chemotherapeutic / toxic hazardous waste

5.8 Areas where biohazards are stored (freezers, refrigerators, incubators) are labeled with the biohazard symbol

5.9 Centrifuge safety covers are used for centrifugation

6.1 Waste containers are properly labeled with the hazards identified on the label

6.2 Liquid waste containers are in secondary containers

6.3 Waste containers are closed unless in use and are not overflowing

6.4 Flame arrestors present and are not clogged

6.5 No chemical waste is added to medical waste containers. sharps are in appropriate containers and are closed except when in use

6.6 No more than one waste container is in use per each waste stream

6.7 Satellite Accumulation Area is indicated with a sign and inspected weekly with documentation

6.8 Satellite Accumulation Area hazardous waste containers are correctly segregated in secondary containment

6.9 Satellite Accumulation Area hazardous waste containers have legible labels that are free of abbreviation

6.10 Full containers are dated and disposed of within 3 working days

6.11 All Non0Regulated Waste streams have legible Non-Regulated waste labels which are free from abbreviations

6.12 There are no flammable waste containers in the Satellite Waste Areas equal to or exceeding 20 L.

6.13 Waste is not actively transferred to HPLC waste containers throughout the day

6.14 No lab waste in regular trash

7.1 Cylinders properly labeled and secured upright to a wall, bench or other stable object

7.2 Cylinder cap in place when gas is not in use

7.3 Cylinders updated in chemical database

7.4 Cylinders are labeled as Empty, Full or In-Use

7.5 Hydrogen cylinders are NOT present in the lab

7.6 Oxygen and carbon dioxide sensors readouts are in the normal range and are calibrated annually

8.1 Electrical cords not frayed and kept neat

8.2 Circuit panes are unobstructed, should have 36" clearance in front and on sides

8.3 Equipment is properly grounded

8.4 No water or chemicals located near outlets

8.5 GFCI cords/outlets in use in areas where there is a potential for moisture to occur

9.1 Hood / BSC sash in appropriate position

9.2 No objects within 6" of face of lab hood or within 4" of face of BSC

9.3 Minimal equipment in hood or BSC

9.4 Surfaces of hoods / weigh stations / BSCs are clean/decontaminated

9.5 Hoods / BSCs / Snorkels are certified and have a Metrology asset tag number

9.6 Unused chemicals are removed from BSC

9.7 BSCs are free of volatile chemicals, flammables, and radioactive materials

9.8 Ceiling vents do not contain visible presence of dust or mold

10.1 Sharps injury logs are maintained for any sharps-related injuries

10.2 Syringes & needles are locked and secured

10.3 Safer sharp devices are used where possible and sharps / blades are used correctly and protected when not in use

10.4 Approved puncture-resistant containers used for sharps waste

10.5 All sharp waste bins (both benchtop and floor standing) are changed out when 3/4 full no sharps are protruding from container or resting on lid

11.1 Cold room is free of hazardous chemicals

11.2 No dry ice or liquid nitrogen is stored in cold room

11.3 No excessive use of any forms of cardboard used in cold rooms (prevents mold growth)

11.4 There is no visible sign of mold present

12.1 The lab has been classified and marked as the appropriate Biosafety level (BSL-1, BSL-2.....)

12.2 Biohazard signs are posted on all entrances to the laboratory

12.3 Laboratory Chemical Inventory List is posted on the entrance door and is updated at the beginning of each quarter.

12.4 A current occupant of the lab is posted as the emergency contact on the laboratory door

12.5 Required PPE is posted on the door of the laboratory

12.6 Current Biosafety Manual and Chemical Hygiene Plan is readily accessible

12.7 GHS Chart is posted in the laboratory