Rodent Survival Surgery Observation

• Survival Surgery Observation

• Surgeon Being Observed:

• PI / Laboratory Location:

• Animal Protocol Number:

• Conducted on

  Date

• Designated IACUC Observer:

Survival Surgery Observation

Preoperative Preparation:

• Have the animal(s) been acclimated to the housing facility at least 48 hours prior to the procedure?

  Yes

  No

  Not Apllicable

• Has the animal(s) health status been assessed before induction with anesthesia?

  Yes

  No

  Not Apllicable

• Is there sufficient supplies of drugs/reagents, sutures, supplies?

  Yes

  No

  Not Apllicable

• Are all drugs/reagents, sutures, and supplies within their expiration date?

  Yes

  No

  Not Apllicable

• Has sufficient time been scheduled to perform the procedure?

  Yes

  No

  Not Apllicable

• Are there any other issue or concerns with preoperative preparation?

  Yes

  No

• List issues or concerns:

Surgery Work Area:

• Is the surgery work space located in the OAR vivarium?

  Yes

  No

• Is the surgical work space dedicated to surgery during the procedures?

  Yes

  No

  Not Apllicable

• Is the surgical work space located away from traffic, air ducts, and windows?

  Yes

  No

  Not Apllicable

• If the surgical work space is a lab bench, are signs posted to warn others not to enter the area during the procedure?

  Yes

  No

  Not Apllicable

• Is the surgical area clean and uncluttered?

  Yes

  No

  Not Apllicable

• Has the surgical space been disinfected?

  Yes

  No

  Not Apllicable

• Have sterile drapes been placed over the surgical work area?

  Yes

  No

  Not Apllicable

• Has a sterile field been created for surgical tools and supplies?

  Yes

  No

  Not Apllicable

• Are all supplies and instruments ready to use and easily available?

  Yes

  No

  Not Apllicable

Instrument/Equipment Sterilization:

• Are instruments cleaned prior to sterilization?

  Yes

  No

  Not Apllicable

• What method is us to sterilize equipment and instruments?

  Select Steam EtO Chemical

  • Steam
  • EtO
  • Chemical

• Are instruments double wrapped?

  Yes

  No

  Not Apllicable

• Is an internal integration strip used to verify sterility?

  Yes

  No

  Not Apllicable

• Was a temperature sensitive indicator used on the outside of the pack?

  Yes

  No

  Not Apllicable

• Internal integration strips are required. External temperature indicators are recommended.

• Was the pack labeled with the sterilization date?

  Yes

  No

  Not Required

• If not used within 24 hours, was the pack stored to prevent exposure to moisture and dust?

  Yes

  No

  Not Required

• Were IACUC guidelines followed for length of exposure to chemical sterilants?

  Yes

  No

  Not Apllicable

• Describe chemical sterilization process:

• If multiple animals are undergoing the surgical procedure in one day, how are instruments resterilized in between animals?

  Dry Bead Sterilizer

  Re-autoclave between animals

  Separate Sets of Tools

• Are instruments cleaned before being re-sterilized?

  Yes

  No

  Not Required

Animal Preparation:

• Is a pre-anesthetic regime required?

  Yes

  No

  Not Required

• Describe the pre-anesthetic regime:

• Is preoperative analgesia given?

  Yes

  No

  Not Required

• Is the anesthetic regime adequate for the procedure?

  Yes

  No

  Not Apllicable

• Is ophthalmic ointment applied to eyes prior to surgery?

  Yes

  No

  Not Required

• Is the surgical site shaved, and the loose hair removed?

  Yes

  No

  Not Required

• It's should not be done on the surgical work space.

• Is the transfer to the surgical work space done sterially?

  Yes

  No

  Not Required

• Is a surgical scrub of the incision site performed?

  Yes

  No

  Not Required

Surgeon Preparation:

• Is surgical scrubs/gowns/lab coat worn?

  Yes

  No

  Not Required

• Is a surgical mask worn?

  Yes

  No

  Not Required

• Is a surgical bonnet/cap worn?

  Yes

  No

  Not Required

• Are gloves put on sterilely?

  Yes

  No

  Not Required

• Did the surgeon (and the assistant of applicable) wash their hands thoroughly before putting on their gloves?

  Yes

  No

  Not Required

Aseptic Surgical Technique

• Was the area around the surgical incision site covered with sterile drapes?

  Yes

  No

  Not Required

• We're instruments and tools unwrapped without compromising sterility?

  Yes

  No

  Not Required

• Did sterile gloves or tools/instruments touch non-sterile items?

  Yes

  No

  Not Apllicable

• Were sutures used to close the wound?

  Yes

  No

  Not Required

• Were internal sutures required?

  Yes

  No

  Not Required

• Were absorbable sutures used?

  Yes

  No

  Not Required

• What type of suture pattern was used?

  Interrupted

  Continuous

  Other

• Describe other suture pattern:

• We're external sutures required?

  Yes

  No

  Not Required

• Were non-absorbable sutures used?

  Yes

  No

  Not Required

• What type of suture pattern was used?

  Buried subcuticular

  Interrupted

  Other

• Describe the other suture patter used?

• Was the sterile suture material maintained in the sterile field at all times?

  Yes

  No

  Not Required

Supportive Care Of Animals During Anesthesia:

• Is the procedure over 30 minutes?

  Yes

  No

  Not Apllicable

• Were body fluids replaced?

  Yes

  No

  Not Required

• Is normal body temperature maintained?

  Yes

  No

  Not Required

Monitoring and Assessment of Anesthesia:

• Is heart rate monitored and recorded?

  Yes

  No

  Not Required

• Is respiratory rate monitored and recorded?

  Yes

  No

  Not Required

• Is body temperature measured and recorded?

  Yes

  No

  Not Required

• Are hemodynamic parameters assessed and recorded?

  Yes

  No

  Not Required

• Mucous membranes should be moist and pink. Capillary refill should be less than 2 seconds.

Anesthetic Recovery:

• Are animals allowed to recover on a surface free from bedding?

  Yes

  No

  Not Required

• Is bedding covered with a drape, and food and water removed from the cage?

  Yes

  No

  Not Required

• Are animals monitored until they are awake?

  Yes

  No

  Not Required

• Is respiration rate monitored during recovery?

  Yes

  No

  Not Required

• Are the animals kept warm?

  Yes

  No

  Not Required

• Is the animals hydration assessed?

  Yes

  No

  Not Required

• Is the intubation tube removed?

  Yes

  No

  Not Apllicable

Post-surgical Care:

• Are animals monitored for pain?

  Yes

  No

  Not Required

• Look for changes in posture, appearance, behavior, and/or appetite.

• Is incision site monitored?

  Yes

  No

  Not Required

• For heat around incision site

• For excessive swelling

• For purulent discharge

• Staples/sutures removed 10-14 days post procedure?

  Yes

  No

  Not Required

Surgical Record Keeping

• Are surgical records maintained for each animal?

  Yes

  No

  Not Required

• Surgical records must include:

• Date

• PI Name

• Surgeon Name

• AP Number

• Animal ID

• Species

• Weight

• Sex

• Age

• Procedure

• Pre-anesthetic Agents

• Anesthetic Agents

• Time of Induction

• Time of Recovery

• Date/Time of Analgesia Administration

• Date of Suture Removal

• Monitoring Observations

• Observations should include date, time, observations, health status

• Monitoring Schedule

• Surgical records are considered complete when the post-op regime is finished, and the sutures are removed

• Every 30 minutes until able to maintain an upright position.

• Daily for the first 5 days post-op

• Every third day after the first 5 days until sutures are removed

Evaluation of Surgeon

• Is the surgeon approved for survival surgery?

  Passed

  Failed

Comments:

• Evaluation/Comments