• Is surgical site accessible for inspection?

  • Contact Gwen Waddingham (384-4773) or Mary Thomson (353-5881) to have them contact the PI to get access.

IACUC Surgical Site Inspection

Surgical/Procedure Area:

  • Is the procedure area for survival surgery, non-survival surgery, or both?

  • Is the surgical space dedicated for animal procedures?

  • Is the space used only for surgery when animals are undergoing procedures?

  • Is the site free from drafts, windows, and laboratory traffic?

  • Is the site free of bedding, animal waste, garbage, clutter, and animal hair?

  • Have dirty cages been removed?

  • Are all surfaces non-porous and washable?

  • Is the storage of food and water for animals, supplies, animal carcasses, and haz mats appropriate?

  • Is there a sharps container present?

  • Is there a biohazard waste container (with and autoclavable liner) present?

  • Are all work surfaces clean, and can they be disinfected?

  • Are disinfectants appropriate and within their expiration dates?

  • Are computers and other equipment covered when not in use?

  • Is there evidence that aseptic technique is used?

  • Do supplies and equipment appear to be clean?

  • Are volatile anesthetics used?

  • What is the method of administration of volatile anesthetics?

  • Is the drop jar used for surgeries?

  • The drop jar method of Isoflurane administration is not allowed for surgical procedures.

  • Has/have the vaporizer(s) been checked/calibrated within the last year?

  • Are anesthetic gases scavenged?

  • How are anesthetic gases scavenged?

  • Are scavenging canisters labeled with initial date of use and weight?

  • Is scavenging canister use recorded by logging the date used and the weight?

  • List the number of vaporizers that are present:

Instrument/Equipment Sterilization:

  • Are instruments and equipment cleaned prior to sterilization?

  • How are instruments, equipment, and supplies sterilized?

  • Was the appropriate procedure followed for duration of exposure and removal sterilizing chemical?

  • Are instruments/tools double wrapped?

  • Are internal integrator strips used?

  • Do instruments and tools have the sterilization date on the packs?

  • Are sterilized instruments and tools stored to prevent exposure to moisture and dust?

  • If multiple animals undergo surgery in one day, how are instruments re-sterilized between animals?


  • Are all anesthetics, analgesics, antibiotics, and other drugs and reagents within their expiration dates?

  • Are all sutures within their expiration date?

  • Are other supplies within their expiration date?

  • Are all controlled substances stored appropriately?

  • Is controlled substance use being recorded/logged appropriately?

  • Are all anesthetics, analgesics, antibiotics, other drugs and reagents pharmaceutical grade?

  • Surgical site inspection is complete.

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