• Is surgical site accessible for inspection?

  • Contact IACUC Office to have them contact the PI to get access.

IACUC Xenopus Surgical Site Inspection

Surgical/Procedure Area:

  • Is the procedure area for survival surgery, non-survival surgery, or both?

  • Is the surgical space dedicated for animal procedures?

  • Is the space used only for surgery when animals are undergoing procedures?

  • Is the site free from drafts, windows, and laboratory traffic?

  • Have dirty cages been removed?

  • Is there a biohazard waste container (with an autoclavable liner) present?

  • Is there a sharps container present?

  • Are all work surfaces clean, and can they be disinfected?

  • Are disinfectants appropriate?

  • 70% EtOH is not acceptable for survival surgeries.
    Examples of acceptable disinfectants:
    Quaternary Ammonium (Santi Cloths, Quatricide, Rocal, Tec-Surf II
    10% bleach (must be made fresh daily)
    Chlorine dioxide (Clidox, MB-10, check instructions for expiration)
    Glutaraldehydes (Cetylcide, Cide Wipes)
    Phenolics (Lysol, TBQ)
    Chlorhexidine (Nolvasan, Hibiclens)

  • Are disinfectants within their expiration date?

  • Is there evidence that aseptic technique is used?

  • Do supplies and equipment appear to be clean?

  • Are there any additional comments for this section?

  • Additional comments/observations for section

  • Is photographic documentation needed for this section?

  • Photographic documentation of procedure space(s)

  • Photographic documentation item
  • Photographic documentation of procedure spaces

Instrument/Equipment Sterilization:

  • Are instruments and equipment cleaned prior to sterilization?

  • How are instruments, equipment, and supplies sterilized?

  • Was the appropriate procedure followed for duration of exposure and removal sterilizing chemical?

  • Are internal integrator strips used?

  • Required. Autoclave tape on the inside of the pack is not acceptable. It must be a sterilization indicator strip.

  • Do instruments and tools have the sterilization date on the packs?

  • Not required if used within 24 hours.

  • Are sterilized instruments and tools stored to prevent exposure to moisture and dust?

  • If multiple animals undergo surgery in one day, how are instruments re-sterilized between animals?

  • Are there any additional comments for this section?

  • Additional comments/observations for section

  • Is photographic documentation needed?

  • Photographic documentation of sterilization of instruments and supplies

  • Sterilization of instruments/supplies
  • Photographic documentation of sterilization of instruments/supplies

Drugs/Sutures/Reagents:

  • Is Tricaine (MS-222) within expiration date?

  • Guidelines for Tricaine expiration dates:
    Room Temp, brown/dark or foil wrapped container - 3-5 days
    Refrigerator, brown/dark or foil wrapped container - 1 month
    Freezer, brown/dark or foil wrapped container - 1 year

    In powder form, in a brown/dark or foil wrapped container kept in a cool dry place - up to 5 years.

  • Are absorbable sutures used for muscle layer?

  • Note: Types of absorbable sutures include - catgut, chromic cat gut, PGA/Dexon, PDS, Vicryl

  • Are non-absorbable sutures, PDS, or Vicryl used to close skin?

  • Note: types of non-absorbable sutures that are acceptable - Nylon (Ethilon), Polypropylene (Prolene)

  • Are all sutures within their expiration date?

  • Are other supplies within their expiration date?

  • Are all anesthetics, analgesics, antibiotics, other drugs and reagents pharmaceutical grade?

  • Are there any additional comments for this section?

  • Additional comments for drugs/reagents/ sutures section:

  • Is any photographic documentation needed for this section?

  • Is any photographic documentation needed for this section?

  • Drugs/Reagents/Sutures
  • Photographic documentation for Drugs/Reagents/Sutures:

Surgical Records

  • Are surgical records available?

  • Are anesthesia/surgical/post-surgical monitoring records kept for each animal?

  • Are anesthesia/surgical records complete?

  • Check missing information:

  • Date of procedure

  • PI name

  • Animal ID

  • Species

  • Procedure

  • Anesthetic regime used

  • Anesthetic Dose

  • Aesthetic route

  • Time of anesthetic induction

  • Time of recovery from anesthesia

  • Surgery number (i.e. - how many surgeries has animal undergone)

  • Can do up to 5 survival surgeries per frog. The 6th surgery must be a terminal procedure.

  • Side of abdomen used recorded?

  • Note: Must wait a minimum of 2 weeks between surgeries if alternate sides are used? Must wait a minimum of 4 weeks between surgeries if only using one side of the abdomen.

  • Are post-surgical monitoring records available?

  • Are animals post-operatively monitored a minimum of 5 days?

  • Note: Monitoring should include weekends and holidays.

  • Are post-surgical monitoring records complete?

  • Check information that is missing

  • Date

  • Time

  • Surgical site observation

  • Animal attitude

  • e.g. - bright/alert/responsive (BAR), quiet/alert/responsive (QAR), depressed, or something along those lines.

  • Health status

  • Could look at activity (normal vs. decreased swimming and diving) or eating (normal vs. decreased food consumption)

  • Are there additional comments for this section?

  • Additional comments for record keeping section:

  • Is any photographic documentation needed for this section?

  • Is any photographic documentation needed for this section?

  • Anesthesia/Surgical/Monitoring Records
  • Photographic documentation for records section

  • Surgical site inspection is complete.

IACUC Semi-annual Non-USDA Surgical Site Inspection Report

  • Thank you for facilitating the IACUC Semi-annual Non-USDA Surgical Site Inspection of your lab. The Office for Laboratory Animal Welfare (OLAW) requires all laboratories performing either survival or non-survival surgery to be inspected by the IACUC every six months. Thank you for your cooperation in helping us fulfill this mandatory compliance requirement.

    This report is a summary of the surgical site inspection for your laboratory. Any deficiencies will be listed in either the Minor deficiency section or the Major deficiency section. Corrective actions will be given for any deficiencies listed and a deadlines will be given for notifying the IACUC Office that the corrective actions have been completed.

    Please contact the IACUC Training & Compliance Monitor by phone or by e-mail if you have any questions, concerns, or comments.

Minor Deficiencies

  • Are there any minor deficiencies?

  • List Minor Deficiencies:

  • There are no minor deficiencies.

Major Deficiencies

  • Are there any major deficiencies?

  • List Major Deficiencies:

  • There are no major deficiencies.

Corrective Actions

  • Are there any corrective actions?

  • Per OLAW regulations and IACUC Policy, corrective actions for Major Deficiencies that affect the health or safety of the animal and/or the safety of personnel must be completed within a specified time which is to be determined by the Institutional Official (IO), the IACUC Chair, the University of Iowa Attending Veterinarian, the IACUC Office Director, and other OAR Clinical Veterinarians.

    Corrective actions for Minor Deficiencies are to be completed by (insert date) and the IACUC Training & Compliance Coordinator should be contacted by e-mail (iacuc-pam@uiowa.edu). and informed that the corrective actions have been completed.

  • List the corrective actions:

  • There are no corrective actions to complete.

Recommended Actions

  • Are there any recommended actions?

  • List recommended actions:

  • There are no recommended actions.

Comments/Notes

  • Surgical Site Inspection Rating:

  • Comments/Notes

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