Are bee actively entering/exiting the hive?
Are the bees bringing in pollen?
Are there signs of robbing?
Are there signs an animal has been disturbing the hive? (Chew or scratch marks from skunks, racoons, etc.)
Are the bees calm when you open the hive? (An agitated or disorganized colony can indicate: queenslessness, poor weather/inspection timing, or a recent intruder)
Is the brood pattern good?
Are larvae healthy, white, and shiny?
Is royal jelly present in cells with larva?
Is there brood in capped and uncapped cells?
Is there one egg or larvae per cell?
Mites test result
Are ants present?
Are wax moths present?
Is there an unusual number of dead bees?
Is there an odor?
How many frames are "covered" in bees? (Look down into the box and see how many seams between frames are full of bees)
How many frames are being fully or almost fully used for brood?
If in a nectar flow, do the bees have space to store nectar?
Do the bees have an empty comb and/or new frames to build comb where they can store nectar?
Has there been any substantial change in total popularity since last infection