TRI Laboratory Audit Checklist

• 

• Document No.

• Audit Title

• Client / Site

• Conducted on

  Date

• Prepared by

• Location
  Address

• Personnel

Facility

• Is the facility fully enclosed space, walls doors, windows, floors and ceilings?

  Yes

  No

  N/A

• Is the facility free from structural issues which affect containment?

  Yes

  No

  N/A

• Are all surfaces smooth, impermeable to water and resistant to cleaning damage?

  Yes

  No

  N/A

• Are open spaces under benches accessible for cleaning?

  Yes

  No

  N/A

• Are facility doors closed while work is occurring?

  Yes

  No

  N/A

• Are any windows closed and sealed?

  Yes

  No

  N/A

• Does the facility restrict access to only authorised persons?

  Yes

  No

  N/A

• Is there a hands free means of decontaminating hands in the facility?

  Yes

  No

  N/A

• Is backflow prevention installed?

  Yes

  No

  N/A

• Is the owner of the facility authorized to carry out maintenance of the facility?

  Yes

  No

  N/A

Biosafety

• Are there OGTR signs and biohazard stickers affixed to access doors and outside lifts?

  Yes

  No

  N/A

• Is disinfectant suitable for use with the GMO’s available in the facility?

  Yes

  No

  N/A

• Is disinfectant suitable for use with the GMO’s available in the facility in date?

  Yes

  No

  N/A

• Is the hand decontaminant solution within date?

  Yes

  No

  N/A

• Are decontaminant solutions for hoods / benches correctly labeled?

  Yes

  No

  N/A

• Are there BSC class I or II in the facility (please list) and are they within the annual test period?

  Yes

  No

  N/A

• Do centrifuges used for human tissue work have bucket lids?

  Yes

  No

  N/A

• Are their in-line filters on laboratory suction bottles?

  Yes

  No

  N/A

• Are fridges and freezers outside the facility posted with biological hazard symbol?

  Yes

  No

  N/A

• Do all fridges/freezers where GMO's are stored have GMO stickers?

  Yes

  No

  N/A

PPE

• Are staff wearing closed footwear?

  Yes

  No

  N/A

• Are staff wearing PPE while working (gown, gloves, eye protection)?

  Yes

  No

  N/A

• Is there designated storage or hanging space for PPE?

  Yes

  No

  N/A

• Is a UV protective face shield available near each UV source?

  Yes

  No

  N/A

• Is there appropriate PPE available for hot work (gloves, face shield)?

  Yes

  No

  N/A

• Are there gloves available for -80 freezers?

  Yes

  No

  N/A

Chemicals

• Are hazardous substance in use?

  Yes

  No

  N/A

• Are SDS available in hardcopy?

  Yes

  No

  N/A

• Are SDS less than 5 years old?

  Yes

  No

  N/A

• Are Flammables stored in the flammables cupboard?

  Yes

  No

  N/A

• Are corrosives stored in the corrosives cupboard?

  Yes

  No

  N/A

• Are hazardous chemicals appropriately segregated?

  Yes

  No

  N/A

• Are S4 drugs stored in a secure area, and the log book available?

  Yes

  No

  N/A

• Are decanted bottles of chemicals clearly labeled with name and risk phrase/DG diamond?

  Yes

  No

  N/A

• Are all hazardous chemicals stored on shelves <1.5m high?

  Yes

  No

  N/A

Waste

• Is waste properly segregated (autoclave, path waste, general waste)?

  Yes

  No

  N/A

• Are waste bins emptied regularly (ie not overflowing)?

  Yes

  No

  N/A

• Is there a mop, dustpan / brush?

  Yes

  No

  N/A

• Are the autoclaves for decontaminating waste validated monthly and calibrated annually?

  Yes

  No

  N/A

• Are sharps containers available in the laboratory?

  Yes

  No

  N/A

Emergency

• Is there an emergency spill kit with an associated cleanup procedure available?

  Yes

  No

  N/A

• Is there a safety shower?

  Yes

  No

  N/A

• Is there a working eyewash in the facility?

  Yes

  No

  N/A

• Is this equipment in good working condition and checked on a regular basis?

  Yes

  No

  N/A

Cold Rooms

• Are items in cold rooms and fridges adequately sealed and labeled?

  Yes

  No

  N/A

• Are the cold rooms free of cardboard boxes?

  Yes

  No

  N/A

Other

• Do circuit breakers work when tested?

  Yes

  No

  N/A

• Is there a pest control strategy in place?

  Yes

  No

  N/A

• Is there evidence of pest or insect activity inside the laboratory area?

  Yes

  No

  N/A

• Is mobile phone/personal electronic device use kept to a minimum and handled with clean, ungloved hands?

  Yes

  No

  N/A

• Is the laboratory free of non-research plants and animals?

  Yes

  No

  N/A

• Is the laboratory kept free of food and drink?

  Yes

  No

  N/A

• Are gas bottles connected properly and within the date stamp?

  Yes

  No

  N/A

• Is there good lighting?

  Yes

  No

  N/A

• Is there a first aid kit available?

  Yes

  No

  N/A

• Are fire exit signs working and visible?

  Yes

  No

  N/A

• Are fire extinguishers and hoses present and within test?

  Yes

  No

  N/A

• Do the fire boards indicate current fire wardens?

  Yes

  No

  N/A

• Do all phones display the emergency contact numbers?

  Yes

  No

  N/A

• Is the area free from noise hazards?

  Yes

  No

  N/A

• Is ear protection available where required?

  Yes

  No

  N/A

• Are all power cords in good condition?

  Yes

  No

  N/A

• Is the area free of stack plugs/double adapters?

  Yes

  No

  N/A

• Is ergonomic furniture provided and used?

  Yes

  No

  N/A

• Are computers correctly positioned at workstations?

  Yes

  No

  N/A

• Are people correctly seated at workstations?

  Yes

  No

  N/A

• Is manual handling observed and performed correctly?

  Yes

  No

  N/A

• Are fire exits free from obstruction?

  Yes

  No

  N/A

• Is the area free off slip / trip hazards?

  Yes

  No

  N/A

• Is anti-fatigue matting provided where staff stand for long periods?

  Yes

  No

  N/A

• Plant Details (location, Asset #, Test Date, Certificate #)

Plant Item

• BSC

  Fume Hood

  Other

• Hazards

Location

• Room #

• Hazards

Action Item

• Outstanding action item

• Has the item been attended to?

  Yes

  No

  N/A

• Is the DRA in certification and has a current diary?

  Yes

  No

  N/A

• Do all users have a current approved radiation protocol submitted with TRI?

  Yes

  No

  N/A

• Is equipment marked with radiation hazard signs?

  Yes

  No

  N/A

• Is the diary being used to record usage in the DRA?

  Yes

  No

  N/A

• Are the monthly swab/screening tests recorded in the diary?

  Yes

  No

  N/A

• Are blue lab coats being used / available?

  Yes

  No

  N/A

• Does the facility have an anteroom for entry?

  Yes

  No

  N/A

• Are facility boundaries designed to prevent escape of animals?

  Yes

  No

  N/A

• Are drainage exits fitted with barriers to prevent animals entering or escaping?

  Yes

  No

  N/A

• Are openings in wall, ceiling or roof filtered and screened with fine mesh?

  Yes

  No

  N/A

• Is the facility free of animals other than arthropods or aquatic organisms?

  Yes

  No

  N/A

• Is the facility free from plants other than those required to work with arthropods?

  Yes

  No

  N/A

• Are all joints between structural components sealed to prevent escape of arthropods?

  Yes

  No

  N/A

• Any openings in the walls, ceiling or roof, such as air vents, must remain screened with mesh that has an aperture size small enough to prevent the escape of the arthropods being contained in the facility?

  Yes

  No

  N/A

• Does the facility have an anteroom?

  Yes

  No

  N/A

• Does the anteroom and work area have methods to control the escape of arthropods?

  Yes

  No

  N/A

• Are all facility doors self-closing?

  Yes

  No

  N/A

• Are drainage exits fitted with barriers to prevent rodents or any other pests from entering the facility?

  Yes

  No

  N/A

• Are containers of disinfectants clearly labeled with the contents and, where necessary, the expiry date?

  Yes

  No

  N/A

• Are all life stages of arthropods in the facility contained in primary containers that are designed to prevent the escape of the arthropods?

  Yes

  No

  N/A

• Are work benches, surfaces and equipment decontaminated after completion of procedures involving any stage of the life cycle of GMOs that could persist on facility surfaces?

  Yes

  No

  N/A

• Are GM arthropods or arthropods containing GM micro-organisms stored outside the facility? If so are they double contained?

  Yes

  No

  N/A

• Are all primary containers labeled to enable identification of the organisms being contained?

  Yes

  No

  N/A

• Is the facility free from animals or plants other than aquatic organisms housed or reared in the facility?

  Yes

  No

  N/A

• Is the facility floor and any bunding sealed?

  Yes

  No

  N/A

• Is the bunding durable, impervious to water and its joints sealed?

  Yes

  No

  N/A

• Does the facility have a system for the decontamination of liquid effluent from the wash-up of equipment such as primary and secondary containers, filters, nets, and any other equipment?

  Yes

  No

  N/A

• Are drainage exits screened or filtered to prevent the escape of aquatic organisms or ingress of animals and invertebrates?

  Yes

  No

  N/A

• Are the facility floor drainage exits, able to treat effluent before being discharged?

  Yes

  No

  N/A

• Are the aquatic organisms in the facility contained in primary containers?

  Yes

  No

  N/A

• Is Secondary containment provided to retain and decontaminate any spillage of water?

  Yes

  No

  N/A

• Are work benches, surfaces and equipment decontaminated after completion of procedures involving GMOs that could persist on facility surfaces?

  Yes

  No

  N/A

• Yes

  No

  N/A

• Yes

  No

  N/A

Questions

• Lookup people on TOPs for audit area. I am doing the annual audit for this area, is it alright if I come in? Not sure if I have introduced myself, James? You are? Do you have any safety concerns/comments you want to raise while I am here? What are you working on today? That is interesting, mind if I watch for a little bit? How do you find it working here? How do you find the people? Do you get enough access to the equipment you need? Do you feel you get the support you need to work effectively? What do you do if/when…? How do you get rid of that waste/clean up at the end? I have noticed... , why do you have things setup that way? Do you do any work with GMO material, specifically NLRD's? Who handles the records for those in your group? I better press on, thanks for your time. My door is always open if you ever have any questions or concerns.