Animal Facility Induction

• Document No.

• Audit Title

• Conducted on

  Date

• Prepared by

• Location
  Address

Conditions of access to experimental facility

• Induction form

• Ethics documentation

• New database set up and training

• Mindflash online training

• Hands on training

General information

• Welcome, introduction of names

• General description of purpose of facility - high level of animal welfare, health, high quality research

• General description of layout of facility - breeding, processing and research areas

• Explanation of the entry order - including pet rodents and working in other facilities

• Rules around access - only authorised/inducted staff allowed. Visitors require approval. Access granted after online, ethics and induction completed.

• Communication - please contact the Animal Facility Officer or Veterinary Manager for any inquiries at all, do not leave something broken/issues unresolved

• Toilets, change rooms

Safety

• Emergency exits

• Emergency contacts and phone locations

• Autoclaves

• Wet floors

Personal protective equipment

• List of required PPE is on room doors

• Booties when stepping to the room

• Gowns - back of door or in drawer

• PC2 masks - protection from allergens

• Nitrile gloves

Procedure room

• Consumables - what is provided by animal facility

• Cleaning up - personal responsibility

Animal room

• Description of IVC racks and AHUs and how they work

• How to ensure racks are connected to AHUs, noting of alarms

• How to ensure cages are connected to racks

• How to read cage cards and essential information on cards

• Flagging cage cards

• New database description - separate training

• Working spaces - hoods recommended for preventing contamination, benches permitted

• How to observe animal behaviour prior to opening cage

• How to correctly open a cage to prevent decontamination of contents

• How to decontaminate hands with ethanol before touching cage contents

• How to pick up and restrain a mouse

• ID markings - ear marks recommended

• Essential cage contents - food, water, nesting, enrichment

• Where to find clean equipment and supplies

• Services provided by staff - changes, food, water etc. Versus monitoring and treatments by research staff

• Where to find sterilised equipment

• Mouse euthanasia - CO2 - safety, 50% fill/min, expected effects

• Other euthanasia options - other anaesthetics/cervical dislocation

• Further training for specific procedures, other areas

• Cadaver disposal

• Record keeping - euthanasia, procedures, monitoring

• Waste disposal, dirty equipment storage, changing waste bags, cleaning up

End of induction

• Additional services e.g monitoring, injections available upon agreement

• Further training for specific procedures, other areas

• Any further questions/feedback

• Attendees signatures

  Sign

• Attendees signatures (additional)

  Sign