MPI Internal Audit Plant and Invertebrate

• Conducted on

  Date

• Conducted by

• Laboratory Manager (for MPI Compliance)

Documentation

• Has the manual been reviewed within the last year?

  Yes

  No

• What was the date of the last review?

• Has there been changes to the manual since the last Audit?

  Yes

  No

• If there has been changes, have staff been informed?

  Yes

  No

  n/a

• Has the current manual been signed by approved users?

  Yes

  No

• Is the disposal register avaliable and up to date?

  Yes

  No

• Is the plant and invertebrate register available and up to date?

  Yes

  No

• Are the transfer documents available?

  Yes

  No

• Are the approvals avaliable?

  Yes

  No

Audits

• What was the date of the last internal audit round?

• Was the date of the last internal audit approximately 6 months since the last one?

  Yes

  No

• Are copies of internal audits available?

  Yes

  No

• Have all internal audit corrective actions been addressed?

  Yes

  No

  n/a

• What was the date of the last external audit?

• Are copies of the external audit avaliable?

  Yes

  No

• Have all external audit corrective actions been addressed?

  Yes

  No

  n/a

Staff Training

• Has refresher training been conducted within the last year?

  Yes

  No

• What was the date of the last refresher training?

• Are training records available for last refresher training?

  Yes

  No

Signage

• Does the building signage conform to Appendix B of AS/NZ 2243.3:2022? (biological hazard symbol, the laboratory containment level, restricted access?)

  Yes

  No

• Does the building signage require any updating?

  Yes

  No

• Are the PC2 work procedures posted in the laboratory?

  Yes

  No

• Are the PC2 work procedures document controlled copies?

  Yes

  No

Laboratory Environment

• Is access to the laboratory restricted?

  Yes

  No

• Is there a visitor log present?

  Yes

  No

• Are all the windows closed and sealed?

  Yes

  No

• Laboratory is free of escape routes for insects?

  Yes

  No

• Are there dedicated hand basins or an alternative means of decontaminating hands provided?

  Yes

  No

• Is the basin mixer for handwashing electronically operated?

  Yes

  No

  n/a

• Are there emergency drench showers provided?

  Yes

  No

  n/a

• Are there emergency eye wash stations provided?

  Yes

  No

• Are the bench top surfaces impervious to liquids, and resistant to commonly used reagents and disinfectants?

  Yes

  No

• Are the bench top joints sealed, sealed to end walls and wet areas?

  Yes

  No

• Are the textured ceilings easily cleanable?

  Yes

  No

• Are the bench top surfaces able to withstand heat generated by general laboratory procedures? eg flaming loops and heating of media

  Yes

  No

  n/a

• Are the floors coved to the walls, smooth, impervious to liquids and easy to clean?

  Yes

  No

• Are the walls smooth, easy to clean, impermeable to liquids?

  Yes

  No

• Are the internal fittings (lights, ducts. shelving) arranged to minimise horizontal dust-collecting surfaces and are able to be cleaned?

  Yes

  No

• Is the laboratory furniture ergonomically suitable?

  Yes

  No

• Is the laboratory furniture seats made of smooth impervious material?

  Yes

  No

• Is the laboratory mechanically ventilated?

  Yes

  No

• Is the directional air-flow into the laboratory maintained?

  Yes

  No

• Does recirculation into the area outside the PC2 facility occur?

  Yes

  No

• Are there lab coats and over-boots provided?

  Yes

  No

• Insect traps are positioned over plants?

  Yes

  No

  n/a

• Are the UV lights functioning?

  Yes

  No

• Have there been any containment breaches since the last audit?

  Yes

  No

• When was the last time the sink sediment trap was emptied?

• Is the virkon within its expiry date?

  Yes

  No

• Are there open spaces under the benches accessible for cleaning?

  Yes

  No

• Are there any carboard boxes on the floor

  Yes

  No

Equipment

• Is the servicing of the Autoclave up to date?

  Yes

  No

• Is there a copy of the servicing report for the autoclave available?

  Yes

  No

• Is Validation of the Autoclave up to date?

  Yes

  No

• Is there a copy of the validation report available?

  Yes

  No

• Is the servicing of the air conditioners up to date?

  Yes

  No

• Is there a copy of the air conditioner report available?

  Yes

  No

Vermin

• Are there any signs of vermin in laboratories?

  Yes

  No

• When was the last vermin control check (Electronic Record through Site)

• Is there a copy of the vermin control check available?

  Yes

  No

Sign Off - Signature and Date

• DFO

• Lab Manager (for MPI Compliance)

• Team Leader