Laboratory Safety and Transition and Containment Facility Audit

• Document No.

• Conducted on

  Date

• Prepared by

  Darrell Smith

  Odette Shaw

  Catherine Davis

• Location

  MIMR

  other

Laboratory Type

• Room number:

  Select 1.09 1.07 1.04 1.01 1.10 1.16 2.35 2.34 2.33 2.28 2.30 2.31 2.37 2.38 2.39 2.40 2.23 2.24 2.21 2.20 2.19 2.16 2.47 other

  • 1.09
  • 1.07
  • 1.04
  • 1.01
  • 1.10
  • 1.16
  • 2.35
  • 2.34
  • 2.33
  • 2.28
  • 2.30
  • 2.31
  • 2.37
  • 2.38
  • 2.39
  • 2.40
  • 2.23
  • 2.24
  • 2.21
  • 2.20
  • 2.19
  • 2.16
  • 2.47
  • other

• Group Leader Responsible for the laboratory

  Select Mike Berridge Elizabeth Forbes-Blom Jacquie Harper Ian Hermans Graham LeGros Kylie Price Franca Ronchese Darrell Smith

  • Mike Berridge
  • Elizabeth Forbes-Blom
  • Jacquie Harper
  • Ian Hermans
  • Graham LeGros
  • Kylie Price
  • Franca Ronchese
  • Darrell Smith

• Type of lab

  Select PC1 PC2 s33 exemption GMP Store

  • PC1
  • PC2
  • s33 exemption
  • GMP
  • Store

• General procedures

  Select GMP Tissue culture Freezer room Flow Cytometry Microscope Chemical preparation Storage Autoclave

  • GMP
  • Tissue culture
  • Freezer room
  • Flow Cytometry
  • Microscope
  • Chemical preparation
  • Storage
  • Autoclave

• Types of material used in the laboratory

  Select Hazardous chemicals Infectious materials Human infectious materials Tissue culture materials Radioactive materials

  • Hazardous chemicals
  • Infectious materials
  • Human infectious materials
  • Tissue culture materials
  • Radioactive materials

Laboratory Facilities

• HSNO exempt laboratory sign on door & contact names current?

  Yes

  No

  N/A

• PC2 and biohazard signage on entry door(s)?

  Yes

  No

  N/A

• Sign in laboratory giving PC2 work procedures

  Yes

  No

  N/A

• First aid kit in vicinity

  Yes

  No

  N/A

• Spill kit available

  Yes

  No

  N/A

• Emergency drench shower operational?

  Yes

  No

  N/A

• Hooks for laboratory coats provided adjacent to exit door?

  Yes

  No

  N/A

• Hands-free hand basin at laboratory exit?

  Yes

  No

  N/A

• Washbasins with hot and cold water operational?

  Yes

  No

  N/A

• Are there spare paper towels and soap available?

  Yes

  No

  N/A

• Emergency eye-wash stations provided?

  Yes

  No

  N/A

• Bench top surfaces impervious? Resistant to solvents? Able to withstand heat?

  Yes

  No

  N/A

• Walls and ceilings smooth, easy to clean, impervious to liquids?

  Yes

  No

  N/A

• Floors non-slip, smooth, easy to clean, impervious to liquids?

  Yes

  No

  N/A

• Laboratory furniture - ergonomic and impervious to liquids?

  Yes

  No

  N/A

• Protective covers provided for keyboards on workbenches?

  Yes

  No

  N/A

• Laboratory mechanically ventilated? Recirculating into area outside PC2 facility does not occur

  Yes

  No

  N/A

• No internal fittings (lights, ducts) damaged

  Yes

  No

  N/A

• Containers for infectious materials and supply of clearly labelled disinfectants for decontamination purposes available

  Yes

  No

  N/A

• A Class 1 or Class 2 biological safety cabinet is available

  Yes

  No

  N/A

• Biological safety cabinet is inspected at least annually by qualified inspector

  Yes

  No

  N/A

• Date certified

  Date

• Fume hood cupboard is fully operational and has current certification?

  Yes

  No

  N/A

• Date certified

  Date

• Biosecurity Safety Cabinet Class II - current certification?

  Yes

  No

  N/A

• Date certified

  Date

• Autoclave - current certification?

  Yes

  No

  N/A

• Date certified

  Date

• Centrifuge fitted with sealed rotors or safety cups is used if large volumes or high concentrations of infectious material used

  Yes

  No

  N/A

• Portable electrical equipment - current PAT certification?

  Yes

  No

  N/A

Work practices

• Protective clothing worn in laboratory

  Yes

  No

  N/A

• No food or drink present?

  Yes

  No

  N/A

• Cultures clearly identified, labelled and appropriately stored

  Yes

  No

  N/A

• All hazardous chemicals clearly identified, labelled and appropriately stored

  Yes

  No

  N/A

• All concentrations visible and suitable warnings clear

  Yes

  No

  N/A

• Chemical data sheets for all chemicals in the laboratory

  Yes

  No

  N/A

• Appropriate procedures for disposal of hazardous chemical

  Yes

  No

  N/A

• Appropriate procedures for disposal of cultures in place

  Yes

  No

  N/A

• Appropriate procedures for disposal of sharps, broken glass

  Yes

  No

  N/A

• No mouth pipetting

  Yes

  No

  N/A

• Laboratory equipment maintained in a clean state (water baths, incubators, Class 2 cabinets, balances, centrifuge, pipettes)

  Yes

  No

  N/A

• No units (including fridge and freezers) outside of designated laboratory used for storage of organisms or DNA posted with biological hazard symbol

  Yes

  No

  N/A

• Open spaces, including under bench are clear and uncluttered?

  Yes

  No

  N/A

• Fridges and Freezers are labelled with "infectious materials" signs?

  Yes

  No

  N/A

• Safe means available to reach items above shoulder height?

  Yes

  No

  N/A

• Laboratory doors are normally closed when work is in progress

  Yes

  No

  N/A

• Class 1 or Class 2 cabinets used for procedures that may generate aerosols, such as shaking, mixing and ultrasonic disruption

  Yes

  No

  N/A

• All material of human origin handled in Class 2 cabinet

  Yes

  No

  N/A

• All microbiological waste autoclaved before disposal

  Yes

  No

  N/A

• All contaminated glassware autoclaved or chemically disinfected prior to washing

  Yes

  No

  N/A

Health and Safety/ Miscellaneous

• Authorised users have received a laboratory orientation/safety briefing and it has been recorded?

  Yes

  No

  N/A

• Fire extinguishers available and close to work area?

  Yes

  No

  N/A

• Date of last fire extinguisher check:

  Date

• Fire blankets available?

  Yes

  No

  N/A

• Lab coats, safety glasses and closed shoes observed?

  Yes

  No

  N/A

Summary Of Inspection

• Any additional items not covered in the audit list:

• Summary of Inspection Outcomes

• The lab meets the requirements for the MIMR HSNO Exempt Laboratory Status?

  Yes

  No

  N/A

• This lab meets the requirements for the MIMR Transition and Containment Facility (PC2)

  Yes

  No

  N/A

• Is a follow-up inspection required?

  Yes

  No

  N/A

• Action needed or results of follow-up

• Laboratory Auditor

  Sign