Materials or agents used:


Used daily?

Bloodborne pathogen and/or Tuberculosis training required?

Infectious shipping training required?

Hepatitis B vaccine recommended?

Advised and trained for specific hazards/procedures?

Engineering Controls

Hand washing facilities?

Readily available with appropriate (antimicrobial) soap?


Personal Protective Equiment

Disposable (single use) gloves available and in use?

Gloves are not worn outside of laboratory

Lab coats or gowns?

Protective eyewear and face shields?


Removed before leaving work area?

Cleaned by professional laundry service?

Work Practice Controls

No eating, drinking, smoking, applying cosmetics, or handling contact lenses in laboratory?

Food or drink is not stored in areas where potentially infectious materials are present?

Appropriate pipetting devices in use?


Policies are in place for the safe handling of sharps, such as needles, scalpels, pipettes, and broken glassware.

Are needles used?

Safe devices in use?

Are there procedures where safer devices cannot be used?

Needle recapping using approved methods?

Are sharps boxes available within arms reach, in sight and routinely replaced when 3/4 full?

Have glass capillary tubes and vacuum tubes been replaced by plastic?

Have blood drawing personnel been advised not to reuse blood tube holders?

Has the practice of injecting blood through a stopper into a vacuum tube using an exposed needle been discontinued?

Work Areas

Absorbent paper in use?


Work surfaces are decontaminated after completion of work and after any spill or splash of potentially infectious material?

Biohazard Postings




Waste containers

Emergency response procedures

Biological Safety Cabinets

Biosafety Cabinets?


Used when potential for aerosols exist?

Clear of all nonessential equipment?

Shared with others

Biohazardous waste

Liquid and solid waste is decontaminated or sterilized before disposal using an effective method?

Autoclaved prior to disposal?

Autoclave SOP and verification of biological effectiveness log?

Containers clearly labeled and covered?

Sharps and broken glassware?

Mechanical devices available for broken glass?