Biosafety Inspection

• Principal Investigator

• Department

• Location
  Address

• Phone

• Conducted on

  Date

• Prepared by

• Materials or agents used:

  Select Recombinant DNA Transgenic organisms Viral or host systems vectors Infectious Agents; bacteria, virus, fungus, prion, etc. Biotoxins, allergens or other biohazardous material Human/non-human primate blood, serum or fluid Human/non-human primate tissue or cell lines

  • Recombinant DNA
  • Transgenic organisms
  • Viral or host systems vectors
  • Infectious Agents; bacteria, virus, fungus, prion, etc.
  • Biotoxins, allergens or other biohazardous material
  • Human/non-human primate blood, serum or fluid
  • Human/non-human primate tissue or cell lines

• Personnel

  Yes

  No

  N/A

• Used daily?

  Yes

  No

  N/A

• Bloodborne pathogen and/or Tuberculosis training required?

  Yes

  No

  N/A

• Infectious shipping training required?

  Yes

  No

  N/A

• Hepatitis B vaccine recommended?

  Yes

  No

  N/A

• Advised and trained for specific hazards/procedures?

  Yes

  No

  N/A

Engineering Controls

• Hand washing facilities?

  Yes

  No

  N/A

• Readily available with appropriate (antimicrobial) soap?

  Yes

  No

  N/A

• Eyewash/shower?

  Yes

  No

  N/A

Personal Protective Equiment

• Disposable (single use) gloves available and in use?

  Yes

  No

  N/A

• Gloves are not worn outside of laboratory

  Yes

  No

  N/A

• Lab coats or gowns?

  Yes

  No

  N/A

• Protective eyewear and face shields?

  Yes

  No

  N/A

• Masks/respirators?

  Yes

  No

  N/A

• Removed before leaving work area?

  Yes

  No

  N/A

• Cleaned by professional laundry service?

  Yes

  No

  N/A

Work Practice Controls

• No eating, drinking, smoking, applying cosmetics, or handling contact lenses in laboratory?

  Yes

  No

  N/A

• Food or drink is not stored in areas where potentially infectious materials are present?

  Yes

  No

  N/A

• Appropriate pipetting devices in use?

  Yes

  No

  N/A

Sharps

• Policies are in place for the safe handling of sharps, such as needles, scalpels, pipettes, and broken glassware.

  Yes

  No

  N/A

• Are needles used?

  Yes

  No

  N/A

• Safe devices in use?

  Yes

  No

  N/A

• Are there procedures where safer devices cannot be used?

  Yes

  No

  N/A

• Needle recapping using approved methods?

  Yes

  No

  N/A

• Are sharps boxes available within arms reach, in sight and routinely replaced when 3/4 full?

  Yes

  No

  N/A

• Have glass capillary tubes and vacuum tubes been replaced by plastic?

  Yes

  No

  N/A

• Have blood drawing personnel been advised not to reuse blood tube holders?

  Yes

  No

  N/A

• Has the practice of injecting blood through a stopper into a vacuum tube using an exposed needle been discontinued?

  Yes

  No

  N/A

Work Areas

• Absorbent paper in use?

  Yes

  No

  N/A

• Disinfectants:

  Select 1% bleach 10% bleach 70% ethanol Dispatch Clidox Quatricide other

  • 1% bleach
  • 10% bleach
  • 70% ethanol
  • Dispatch
  • Clidox
  • Quatricide
  • other

• Work surfaces are decontaminated after completion of work and after any spill or splash of potentially infectious material?

  Yes

  No

  N/A

Biohazard Postings

• Door

  Yes

  No

  N/A

• Refrigerators/Freezers

  Yes

  No

  N/A

• Equipment

  Yes

  No

  N/A

• Waste containers

  Yes

  No

  N/A

• Emergency response procedures

  Yes

  No

  N/A

Biological Safety Cabinets

• Biosafety Cabinets?

  Yes

  No

  N/A

• Type

• Used when potential for aerosols exist?

  Yes

  No

  N/A

• Clear of all nonessential equipment?

  Yes

  No

  N/A

• Shared with others

Biohazardous waste

• Liquid and solid waste is decontaminated or sterilized before disposal using an effective method?

  Yes

  No

  N/A

• Autoclaved prior to disposal?

  Yes

  No

  N/A

• Autoclave SOP and verification of biological effectiveness log?

  Yes

  No

  N/A

• Containers clearly labeled and covered?

  Yes

  No

  N/A

• Sharps and broken glassware?

  Yes

  No

  N/A

• Mechanical devices available for broken glass?

  Yes

  No

  N/A

• Comments/recommendations